AmfS, an extracellular peptidic morphogen in Streptomyces griseus
AmfS, an extracellular peptidic morphogen in Streptomyces griseus
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DOI：
10.1128/jb.184.5.1488-1492.2002
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发表时间：
2002-03-01
影响因子：
3.2
通讯作者：
Beppu, T
中科院分区：
文献类型：
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作者：
Ueda, K;Oinuma, KI;Beppu, T
在灰色链霉菌中，AMF基因簇被认为是气生菌丝体形成的调节因子。AMF的核苷酸序列与其他物种的同源序列显示了一个由5个开放阅读框组成的保守的基因组织。编码43个氨基酸的AMFS的无义突变导致气生菌丝的形成和链霉素的产生显著受阻，这表明它是一种调节分子。AMFS突变株气生菌丝体缺失表型的胞外互补试验证明，AMFS是由野生型菌株分泌的。编码HlyB样膜转运子的amfBA零突变在不影响野生型形态的情况下取消了胞外AMFS的活性，这表明AmfBA不参与AMFS的生产，而参与AMFS的出口。在AMFS突变体中，合成的C末端八肽部分诱导了气生菌丝体的形成，这表明AMFS衍生物而不是AMFS本身是胞外形态因子。
The amf gene cluster was previously identified as a regulator for the onset of aerial-mycelium formation in Streptomyces griseus. The nucleotide sequences of amf and its counterparts in other species revealed a conserved gene organization consisting of five open reading frames. A nonsense mutation in amfS, encoding a 43-amino-acid peptide, caused significant blocking of aerial-mycelium formation and streptomycin production, suggesting its role as a regulatory molecule. Extracellular-complementation tests for the aerial-mycelium-deficient phenotype of the amfS mutant demonstrated that AmfS was secreted by the wild-type strain. A null mutation in amfBA, encoding HlyB-like membrane translocators, abolished the extracellular AmfS activity without affecting the wild-type morphology, which suggests that AmfBA is involved not in production but in export of AmfS. A synthetic C-terminal octapeptide partially induced aerial-mycelium formation in the amfS mutant, which suggests that an AmfS derivative, but not AmfS itself, serves as an extracellular morphogen.