Hemopoietic progenitors in the murine fetal liver capable of rapidly generating T cells.
Hemopoietic progenitors in the murine fetal liver capable of rapidly generating T cells.
复制标题
小鼠胎肝中的造血祖细胞能够快速生成 T 细胞。
DOI：
10.4049/jimmunol.158.7.3118
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发表时间：
1997
影响因子：
4.4
通讯作者：
Yoshimoto Katsura
中科院分区：
文献类型：
--
作者：
Hiroshi Kawamoto;K. Ohmura;Noboru Hattori;Yoshimoto Katsura
我们研究了胎儿肝脏 (FL) 中的造血祖细胞与成人骨髓 (BM) 中的造血祖细胞分化为 T、B 和骨髓细胞的能力。从 FL 和 BM 分离的谱系标记阴性 (Lin-) c-kit+ 细胞用作祖细胞的来源。当 FL 和 BM 祖细胞在 BM 衍生的基质细胞系的单层上培养时，在生成 B 细胞和骨髓细胞的能力方面没有观察到明显差异。与此形成鲜明对比的是，在体内胸腺细胞转移和胎儿胸腺器官培养系统中，FL 祖细胞能够比 BM 祖细胞更快地生成 T 细胞。在先天性无胸腺 nu/nu 小鼠的 FL 中也发现了相同类型的 FL 祖细胞，表明这些祖细胞在 FL 中产生，独立于胎儿胸腺。本研究提供了间接证据，表明 FL 中存在 T 细胞谱系定向祖细胞。
We investigated the hemopoietic progenitors in fetal liver (FL) compared with those in adult bone marrow (BM) for their ability to differentiate into T, B, and myeloid cells. Lineage marker-negative (Lin-) c-kit+ cells isolated from FL and BM were used as the source of progenitors. No apparent difference was observed between FL and BM progenitors in their ability to generate B and myeloid cells when these progenitors were cultured on a monolayer of a BM-derived stromal cell line. In marked contrast, FL progenitors were able to generate T cells much more rapidly than BM progenitors in both in vivo intrathymic cell transfer and fetal thymus organ culture systems. The same type of FL progenitors were also found in the FL of congenitally athymic nu/nu mice, indicating that these progenitors arise in FL independently of the fetal thymus. The present study have provided circumstantial evidence suggesting the presence of T cell lineage-committed progenitors in FL.