Characterization and comparative expression of zebrafish calpain system genes during early development
Characterization and comparative expression of zebrafish calpain system genes during early development
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DOI：
10.1002/dvdy.21459
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发表时间：
2008-03-01
影响因子：
2.5
通讯作者：
Bruce, Ashley E. E.
中科院分区：
文献类型：
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作者：
Lepage, Stephanie E.;Bruce, Ashley E. E.
经典的钙蛋白酶系统已被牵连在调节各种细胞过程，包括细胞粘附，迁移和细胞内信号转导，然而，很少有人知道关于这个系统在体内的功能。两种异源二聚体Ca 2+依赖性半胱氨酸蛋白酶mu-calpain（CAPN 1）和m-calpain（CAPN 2）以及内源性抑制剂calpastatin（CAST）构成了哺乳动物中经典/普遍存在的钙蛋白酶系统。最近，敲除两个小鼠经典的钙蛋白酶基因，Capn 2和Capn 4/Capns 1，揭示了经典系统的组件是不可缺少的植入前发育。我们从斑马鱼中鉴定了4个经典的钙蛋白酶催化亚基基因（capn 1a，1b，2a，2b），2个调节亚基基因（capns 1a，1b）和钙蛋白酶抑制蛋白（cast）。我们的数据表明，经典的哺乳动物系统的组成部分是保守的，并扩大了硬骨鱼谱系。与经典/普遍存在的哺乳动物系统相反，斑马鱼钙蛋白酶系统基因在发育的前2天内获得独特的组织特异性表达模式。
The classic calpain system has been implicated in regulating a variety of cellular processes including cell adhesion, migration, and intracellular signaling; however, little is known regarding the function of this system in vivo. Two heterodimeric Ca2+-dependent cysteine proteases, mu-calpain (CAPN1) and m-calpain (CAPN2), and the endogenous inhibitor calpastatin (CAST) comprise the classic/ubiquitous calpain system in mammals. Recently, knockout of two murine classic calpain genes, Capn2 and Capn4/Capns1, revealed that components of the classic system are indispensable for preimplantation development. We identified four classic calpain catalytic subunit genes (capn1a, 1b, 2a, 2b), two regulatory subunit genes (capns1a, 1b), and calpastatin (cast) from the zebrafish. Our data suggest that the components of the classic mammalian system are both conserved and expanded in the teleost lineage. In contrast to the classic/ubiquitous mammalian system, zebrafish calpain system genes acquire unique, tissue-specific patterns of expression within the first 2 days of development.