Transcription of a novel P450 gene varies with some factors (pollutant exposure, temperature, time, and body region) in a marine oligochaete (Thalassodrilides sp.)
Transcription of a novel P450 gene varies with some factors (pollutant exposure, temperature, time, and body region) in a marine oligochaete (Thalassodrilides sp.)
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在海洋寡毛类动物（Thalassodrilides sp.）中，新型 P450 基因的转录随某些因素（污染物暴露、温度、时间和身体区域）的变化而变化。
DOI：
10.1016/j.marpolbul.2016.05.055
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发表时间：
2016
影响因子：
5.8
通讯作者：
Kazuhiko Mochida
中科院分区：
文献类型：
--
作者：
Mana Ito;Katsutoshi Ito;Kohei Ohta;Takeshi Hano;Toshimitsu Onduka;Kazuhiko Mochida
细胞色素P450 （CYP）酶在多环芳烃（PAHs）等外源化合物的代谢中起重要作用。一种新的全长CYP基因（CYP4V30）在少角藻海鞘中被鉴定出来。在暴露于多环芳烃污染（Σ16PAHs； 37441 ng/g干重）或未污染（Σ16PAHs； 19 ng/g干重）的沉积物中研究CYP4V30mRNA的表达。在某些温度（20°C和25°C）和暴露时间（20°C， 10天）下，暴露于污染沉积物的蠕虫cyp4v30表达量远高于未污染沉积物的蠕虫，但在15°C或其他暴露时间（P< 0.05）下没有表达（P< 0.05）。CYP4V30mRNA在体中部的表达高于后部（P< 0.05）。转录响应随暴露时间、温度和身体区域的变化表明，在监测海洋沉积物污染时应考虑这些因素。
Cytochrome P450 (CYP) enzymes play important roles in the metabolism of exogenous compounds such as polycyclic aromatic hydrocarbons (PAHs). A novel, full-length CYP gene (CYP4V30) was identified in the oligochaeteThalassodrilidessp.CYP4V30mRNA expression was studied in worms exposed to PAH-polluted (Σ16PAHs; 37441 ng/g dry weight) or unpolluted (Σ16PAHs; 19 ng/g dry weight) sediment.CYP4V30expression was much higher in worms exposed to contaminated sediments than in those exposed to unpolluted sediments at some temperatures (20 and 25 °C) and exposure durations (11-fold increase at 20 °C, 10-day exposure), but not at 15 °C or other exposure durations (P< 0.05).CYP4V30mRNA expression was higher in the middle of the body than in the posterior (P< 0.05). The variation in transcriptional response with exposure time, temperature, and body region indicates that these factors should be considered when monitoring marine sediment pollution.