A Novel Insulinotropic Peptide from the Skin Secretions of Amolops loloensis Frog.
A Novel Insulinotropic Peptide from the Skin Secretions of Amolops loloensis Frog.
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DOI：
10.1007/s13659-014-0037-z
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发表时间：
2014-10
影响因子：
4.7
通讯作者：
Rong MQ
中科院分区：
文献类型：
--
作者：
Mo GX;Bai XW;Li ZJ;Yan XW;He XQ;Rong MQ
此前已从罗洛青蛙的皮肤分泌物中分离出多种生物活性肽，如抗菌肽、缓激肽样肽和镇痛肽。在洛洛青蛙的皮肤分泌物中发现了一种名为阿莫洛平的新型促胰岛素肽。通过Edman降解确定其一级结构序列为：FLPIVGKSLSGLSGKL-NH2。 BLAST 搜索表明，amolopin 的氨基酸序列与其他已知的胰岛素促分泌剂（包括 Mastoparan、Exendin 和 α-latrotoxin）有很大不同，也不像肠降血糖素（例如肽-1 和葡萄糖依赖性促胰岛素多肽等胰高血糖素）。然而，阿莫洛平与两栖动物抗菌颞肌蛋白和从 Vespa magnifica 中分离出的胡蜂趋化肽具有一定的结构相似性。 Amolopin 可以以剂量依赖性方式刺激 INS-1 细胞中的胰岛素释放。对其作用机制的初步研究表明，阿莫洛平不会增加 Ca2+ 的流入。总之，首次从罗洛青蛙的皮肤分泌物中发现了一种具有胰岛素释放活性的新型16氨基酸肽。需要进一步的工作来评估其作为新型抗糖尿病候选药物的潜力。
Various kinds of biologically active peptides have previously been isolated from the skin secretions of Amolops loloensis frog, such as antimicrobial peptides, bradykinin-like peptides and algesic peptides. A novel insulinotropic peptide named amolopin was identified in A. loloensis frog’s skin secretion. Its primary structure sequence was determined by Edman degradation as: FLPIVGKSLSGLSGKL-NH2. BLAST search indicates that the amino acid sequence of amolopin is quite different from other known insulin secretagogues, including mastoparan, exendins and α-latrotoxin, nor does it like incretins (e.g. glucagons like peptide-1 and glucose-dependent insulinotropic ploypeptide) either. However, amolopin shows certain structural similarity with amphibian antimicrobial temporins and vespid chemotactic peptides isolated from Vespa magnifica. Amolopin can stimulate insulin release in INS-1 cells in a dose-dependent manner. Primary investigation on its action mechanisms reveals that amolopin does not increase the influx of Ca2+. In conclusion, a novel 16-amino acid peptide with insulin-releasing activity is initially discovered from the skin secretion of A. loloensis frog. Further work is necessary to evaluate its potential as novel anti-diabetic candidate.