CHARACTERIZATION AND OPTIMIZATION OF HYDROGEN PHOTOPRODUCTION BY A SALTWATER BLUE-GREEN-ALGA, OSCILLATORIA SP MIAMI BG7 .1. ENHANCEMENT THROUGH LIMITING THE SUPPLY OF NITROGEN NUTRIENTS
CHARACTERIZATION AND OPTIMIZATION OF HYDROGEN PHOTOPRODUCTION BY A SALTWATER BLUE-GREEN-ALGA, OSCILLATORIA SP MIAMI BG7 .1. ENHANCEMENT THROUGH LIMITING THE SUPPLY OF NITROGEN NUTRIENTS
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DOI：
10.1016/0360-3199(81)90060-4
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发表时间：
1981-01-01
影响因子：
7.2
通讯作者：
MITSUI, A
中科院分区：
文献类型：
--
作者：
KUMAZAWA, S;MITSUI, A
一种海洋非异型囊丝状蓝绿色绿藻颤藻光生氢的研究。研究了迈阿密BG 7与培养基中氮营养的关系。当氮充足的组合培养物接种到氮限制的组合培养基中时，在培养期间，叶绿素和蛋白质的细胞含量降低，而碳水化合物含量显著增加。随着细胞组成的变化，光合产氧能力下降，光合产氢能力显著提高。最大光产氢速率为260 μmol/mg叶绿素/h。在厌氧条件下，产氢严格依赖于光。沿着光生氢，也产生二氧化碳。光生氢和二氧化碳的比例约为2。复合氮限制培养提高光合产氢的机制与培养过程中固氮酶合成增加、光系统II活性下降和电子供体物质积累有关。氢气生产的另一个有趣的特征是在没有任何特殊处理的情况下气体在烧瓶中的积累。这与该菌株中缺乏氢吸收活性有关。这些结果表明，该菌株具有良好的特性，为未来的应用，生物太阳能燃料生产。
Hydrogen photoproduction by a marine, non-heterocystous, filamentous blue-green alga,Oscillatoriasp. Miami BG7 was studied in its relationship to nitrogen nutrients in the culture medium. When a combined nitrogen sufficient culture was inoculated into a combined nitrogen limited medium, cellular content of chlorophyll and protein decreased whereas the carbohydrate content increased significantly during the culture period. Accompanying this change of cellular composition the oxygen photoproduction capability decreased and hydrogen photoproduction capability increased dramatically. The maximum rate of hydrogen photoproduction was 260 μmol/mg chlorophyll/h. Hydrogen production was strictly light dependent under an anaerobic condition. Along with hydrogen photoproduction, carbon dioxide was also produced. The ratio of hydrogen and carbon dioxide photoproduction was approximately two. The mechanism of hydrogen photoproduction enhancement in combined nitrogen limited culture is apparently related to the increase of nitrogenase synthesis, decline of photosystem II activity, and the accumulation of electron donor substances during culture. Another interesting feature of hydrogen production was accumulation of gas in the flasks without any special treatments. This is related to the lack of hydrogen uptake activity in this strain. These results indicate that this strain has favorable characteristics for future application to biosolar fuel production.