Visualization of RNA-Quadruplexes in Live Cells
Visualization of RNA-Quadruplexes in Live Cells
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DOI：
10.1021/jacs.5b03413
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发表时间：
2015-07-08
影响因子：
15
通讯作者：
Monchaud, David
中科院分区：
文献类型：
--
作者：
Laguerre, Aurelien;Hukezalie, Kyle;Monchaud, David
最近用不同的四链体特异性抗体证明了人细胞中DNA和RNA四链体形成的可视化。尽管这些免疫检测方法的显着利益，在活细胞中的四链体的动态检测仍然是难以捉摸的。在这里，我们报告的NaphthoTASQ（N-TASQ），下一代四链体配体，作为一个多光子打开荧光探针。人和小鼠细胞与N-TASQ的单步孵育能够直接检测未处理细胞中的RNA四链体（无固定、透化或封固步骤），从而提供活细胞中四链体的独特、无偏倚的可视化。
Visualization of DNA and RNA quadruplex formation in human cells was demonstrated recently with different quadruplex-specific antibodies. Despite the significant interest in these immunodetection approaches, dynamic detection of quadruplex in live cells remains elusive. Here, we report on NaphthoTASQ (N-TASQ), a next-generation quadruplex ligand that acts as a multiphoton turn-on fluorescent probe. Single-step incubation of human and mouse cells with N-TASQ enables the direct detection of RNA-quadruplexes in untreated cells (no fixation, permeabilization or mounting steps), thus offering a unique, unbiased visualization of quadruplexes in live cells.