Dose-dependent short-term study of di-n-butyl phthalate on the testicular antioxidant system of Wistar rats
Dose-dependent short-term study of di-n-butyl phthalate on the testicular antioxidant system of Wistar rats
复制标题
DOI：
10.1007/s11356-014-3457-8
复制
发表时间：
2015-02-01
影响因子：
5.8
通讯作者：
Nair, Neena
中科院分区：
文献类型：
--
作者：
Nair, Neena
邻苯二甲酸二正丁酯（DBP）是一种外源性物质，在工业中广泛用作聚氯乙烯树脂的增塑剂。本研究的目的是评估DBP是否会诱导Wistar大鼠睾丸产生氧化应激。将剂量为500、1000和1500 mg/kg体重（低于半数致死量的剂量）的DBP经口给予大鼠，持续7天。在最后一次给药24小时后，用乙醚麻醉处死动物。观察到睾丸重量无显著增加。组织学研究表明，生精细胞、睾丸间质细胞和支持细胞出现与剂量相关的变性，同时管腔内精子丢失。丙二醛（TBARS）、脂质氢过氧化物的浓度、水溶性抗氧化能力、谷胱甘肽 - S - 转移酶、过氧化氢酶以及微量元素锌和铜的浓度增加，而总蛋白浓度、脂溶性抗氧化能力、抗坏血酸、谷胱甘肽、总超氧化物歧化酶（SOD）、铜 - 锌超氧化物歧化酶、锰超氧化物歧化酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶和金属硫蛋白在所有剂量水平下均降低。数据表明，由于精子发生受损，细胞功能受到不利影响，这表明存在氧化应激，抗氧化防御系统的改变证明了这一点，而这种改变似乎是通过下丘脑 - 垂体 - 性腺轴介导的。睾丸的变化谱反映出即使在低剂量下，它对邻苯二甲酸酯也很敏感，有可能干扰关键的生殖功能。
Di-n-butyl phthalate (DBP), a xenobiotic, is widely used in industries as a softener for polyvinyl chloride resins. The aim of the present study was to evaluate whether DBP induces oxidative stress in testes of Wistar rats. DBP at doses of 500, 1,000 and 1,500 mg/kg b.wt. (doses below LD50) was given orally for 7 days. After 24 hrs from the last dose, the animals were killed under ether anesthesia. Nonsignificant increase in testicular weight was observed. Histological studies indicated a dose-related degeneration of germinal, Leydig and Sertoli cells along with loss of spermatozoa in the lumen. The concentrations of malondialdehyde (TBARS), lipid hydroperoxides, water-soluble antioxidant capacity, glutathione-S-transferase, catalase and trace elements-zinc and copper increased while concentrations of total protein, lipid soluble antioxidant capacity, ascorbic acid, glutathione, total superoxide dismutase (SOD), Cu-ZnSOD, MnSOD, glutathione peroxidase, glutathione reductase and metallothionein decreased at all the dose levels. The data suggests that the cellular functions were adversely affected due to impairment of spermatogenesis indicative of oxidative stress as evident by altered antioxidative defense system which appears to mediate through hypothalamo-pituitary-gonadal axis. The spectrum of changes in testes reflects its susceptibility to phthalate even at low dose with the potential to interfere with critical reproductive function.