Fatty acid composition in fetal, neonatal, and cultured cardiomyocytes in rats
Fatty acid composition in fetal, neonatal, and cultured cardiomyocytes in rats
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大鼠胎儿、新生儿和培养心肌细胞的脂肪酸组成
DOI：
10.1007/s11626-013-9668-3
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Nakamura T
中科院分区：
文献类型：
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作者：
Karimata T;Seya D;Wakatsuki T;Feng Z;Sato D;Nishina A;Kusunoki M;Nakamura T
重建的心肌组织仍然没有足够的搏动性收缩。众所周知，胎儿和成熟的新生儿心肌细胞分别利用葡萄糖和脂质作为其能量底物，而培养的心肌细胞主要利用葡萄糖，尽管它们的年龄与新生儿心肌细胞相当，这可能是由于培养基中脂质供应不足。在本研究中，我们比较了7饱和，6单不饱和，和11多不饱和脂肪酸含量培养心肌细胞（Cul组）与胎儿（Fet组，约17天后怀孕）和新生儿（Neo组，9天）大鼠，其中Cul细胞的年龄被设置为几乎等于Neo的。Cul组的饱和脂肪酸含量普遍低于Fet组，与Neo组接近，但C12：0含量在Neo组最高。Cul组的单不饱和脂肪酸含量一般低于Fet组，但与Neo组相似或高于Neo组，但C24：1 n-9的含量在Neo组中再次最高。与此相反，Cul组的多不饱和脂肪酸（PUFA）含量大多数低于Fet和Neo组，Cul组和Neo组之间有5种PUFA含量差异显著。结果表明，培养的心肌细胞中PUFA含量可能不足以发挥足够的收缩能力。总之，培养的心肌细胞可能需要摄取更多的脂质，特别是PUFA。
Reconstructed myocardial tissue still does not have enough pulsatile contraction. It is well known that fetal and mature neonatal cardiomyocytes utilize glucose and lipid, respectively, as their energy substrates, and that cultured ones mainly use glucose in spite of their age comparable to neonate ones, probably due to insufficient supply of lipids from culture medium. In the present study, we compared 7 saturated, 6 monounsaturated, and 11 polyunsaturated fatty acid contents in cultured cardiomyocytes (Cul group) with those in fetal (Fet group, approximately 17 d after impregnation) and neonatal (Neo group, 9 d old) rats, where the age of the Cul cells were set nearly equal to the Neo ones. Saturated fatty acid contents in the Cul group were generally lower than those in the Fet group and were close to those in the Neo group, except for C12:0 of which content was highest in the Neo group. Monounsaturated fatty acid contents in the Cul group were generally lower than those in the Fet group but similar to or higher than those in the Neo group, except for C24:1n-9 of which content was again highest in the Neo group. In contrast, most of polyunsaturated fatty acid (PUFA) contents in the Cul group appeared lower than those in both the Fet and Neo groups, and differences in 5 of 10 detected PUFAs were significant between the Cul and Neo groups. The results suggest that PUFA contents in cultured cardiomyocytes might be insufficient to exert enough contractile ability. In conclusion, it could be necessary for cultured cardiomyocytes to uptake more lipid; PUFAs in particular.