Purification of oligonucleotides using denaturing polyacrylamide gel electrophoresis.
Purification of oligonucleotides using denaturing polyacrylamide gel electrophoresis.
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DOI：
10.1002/0471142727.mb0212s42
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发表时间：
2001-05-01
影响因子：
--
通讯作者：
Pollard, J D Jr
中科院分区：
文献类型：
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作者：
Ellington, A;Pollard, J D Jr
来源于丝状噬菌体的克隆载体非常有用，因为它们允许克隆的DNA作为单链或双链DNA分离。该单元包含用于制备两种形式的DNA和用于表征M13衍生载体中的插入物的方案。还提出了使用辅助噬菌体超感染从质粒制备单链DNA的方案。这种方法是有利的，因为它允许克隆的DNA以质粒的形式保持，同时允许分离单链DNA用于DNA测序。
Cloning vectors derived from filamentous phage are extremely useful because they allow cloned DNA to be isolated as either single- or double-stranded DNA. This unit contains protocols for preparing both forms of DNA and for characterizing inserts in M13-derived vectors. A protocol is also presented for preparing single-stranded DNA from plasmids using superinfection with helper phage. This method is advantageous because it allows cloned DNA to be maintained in the form of a plasmid while permitting single-stranded DNA to be isolated for DNA sequencing.