Application of surfactants for controlling destructive fungus contamination in mass cultivation of Haematococcus pluvialis
Application of surfactants for controlling destructive fungus contamination in mass cultivation of Haematococcus pluvialis
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表面活性剂在雨生红球藻大规模培养中控制破坏性真菌污染的应用
DOI：
10.1016/j.biortech.2020.124025
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发表时间：
2020-12-01
影响因子：
11.4
通讯作者：
Li, Yeguang
中科院分区：
文献类型：
--
作者：
Ding, Yi;Zhang, Aoqi;Li, Yeguang
雨生红球藻在规模化培养过程中，经常会受到破坏性真菌--西德伯氏拟葡萄球菌的污染，给虾青素的生产造成巨大损失。在这里，我们建议使用四种商业表面活性剂来控制雨生螺旋藻培养物中的P.sedebokerense污染。在实验室实验中，十二烷基苯磺酸钠、十二烷基硫酸钠和伯醇乙氧基化物处理对真菌污染有抑制作用。此外，十二烷基苯磺酸钠是最有希望的，因为7 mg的L-1处理对雨生螺旋藻的生长和虾青素含量没有负面影响，但对真菌感染有效。这可能归因于它们不同的细胞覆盖物和结构。此外，在露天回旋式池塘中应用十二烷基苯磺酸钠防止了真菌污染，虾青素产量达到1.82g m~(-2)。因此，十二烷基苯磺酸钠可以作为一种经济有效的防腐剂用于雨生红豆杉虾青素的工业化生产。
Large-scale cultivation of Haematococcus pluvialis is frequently contaminated by the destructive fungus Paraphysoderma sedebokerense, which can cause huge losses in astaxanthin production. Here, we propose the use of four commercial surfactants to control P. sedebokerense contamination in H. pluvialis cultures. In laboratory experiments, sodium dodecylbenzene sulfonate, sodium dodecyl sulfate and primary alcohol ethoxylate treatments showed inhibitory effects on fungal contamination. Moreover, sodium dodecylbenzene sulfonate was the most promising because treatment at 7 mg L-1 was effective against fungal infection without negatively affecting the growth or astaxanthin contents of H. pluvialis. This could be ascribed to their different cell coverings and structures. Additionally, applying sodium dodecylbenzene sulfonate to open raceway ponds prevented fungal contamination, and astaxanthin production reached 1.82 g m(-2). Therefore, sodium dodecylbenzene sulfonate can be used as an effective and economical control agent for commercial production of astaxanthin from H. pluvialis.