Noninvasive measurement of cardiovascular function in mice with high-temporal-resolution small-animal PET.
Noninvasive measurement of cardiovascular function in mice with high-temporal-resolution small-animal PET.
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发表时间：
2006-06
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通讯作者：
M. Kreissl;Hsiao-Ming Wu;D. Stout;W. Ladno;T. Schindler;Xiaoli Zhang;John O. Prior;M. Prins;
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作者：
M. Kreissl;Hsiao-Ming Wu;D. Stout;W. Ladno;T. Schindler;Xiaoli Zhang;John O. Prior;M. Prins;
未标记本研究的目的是探索通过专用小动物PET系统的高时间分辨率成像非侵入性地确定小鼠心血管功能参数的可行性。方法25只麻醉小鼠（28.8 ± 4.6 g）经静脉导管注射30 μ L（18）F-FDG（8-44 MBq）。使用滤波反投影将前9秒数据重建为30帧0.3秒。从感兴趣的左心室容积导出的时间-活性曲线针对示踪剂再循环和部分容积进行校正。通过Stewart-Hamilton方法计算心输出量，其中心输出量是总注射活动除以左心室时间-活动曲线下面积。心输出量除以体重定义为心脏指数;心输出量除以心率得到每搏输出量。在5只小鼠中，重复测量2-4次以评估再现性。在4只小鼠中，通过测量心率、心输出量和每搏输出量来检查对多巴酚丁胺的血流动力学反应。结果：心输出量平均为20.4 +/- 3.4 mL/min;在重复测量中，该参数显示每只小鼠的平均SD百分比为10% +/-6%。心脏指数平均为0.73 +/- 0.19 mL/min/g，每搏输出量为45.0 +/- 6.9 microL，两者均与心率相关（分别为r = 0.53，P = 0.007和r = 0.49，P = 0.01）。在多巴酚丁胺应激期间，心率从423 +/- 50增加到603 +/- 30次/分（P = 0.002），心输出量从18.5 +/- 1.9增加到32.0 +/- 4.2 mL/分（P = 0.008）。结论采用高时间分辨率的小动物PET显像技术可以无创性地检测小鼠心血管功能参数。心输出量和每搏输出量的测量值是可重现的，并且与MRI获得的值相当。该方法允许监测心血管功能的变化，以响应药物干预。
UNLABELLED The aim of this study was to explore the feasibility of determining parameters of cardiovascular function in mice noninvasively by high-temporal-resolution imaging with a dedicated small-animal PET system. METHODS Twenty-five anesthetized mice (28.8 +/- 4.6 g) were injected via an intravenous catheter with a 30-microL bolus of (18)F-FDG (8-44 MBq). The first 9 s of data were reconstructed into 30 frames of 0.3 s using filtered backprojection. The time-activity curve derived from a left ventricle volume of interest was corrected for tracer recirculation and partial volume. Cardiac output was calculated by the Stewart-Hamilton method, in which cardiac output is total injected activity divided by the area under the left ventricle time-activity curve. Cardiac output divided by body weight was defined as cardiac index; cardiac output divided by heart rate yielded the stroke volume. In 5 mice, measurements were repeated 2-4 times to assess reproducibility. In 4 mice, the hemodynamic response to dobutamine was examined by measuring heart rate, cardiac output, and stroke volume. RESULTS The cardiac output averaged 20.4 +/- 3.4 mL/min; in the repeated measurements, the parameter displayed a mean percentage SD per mouse of 10% +/- 6%. The cardiac index averaged 0.73 +/- 0.19 mL/min/g and the stroke volume 45.0 +/- 6.9 microL, and both correlated with heart rate (r = 0.53, P = 0.007, and r = 0.49, P = 0.01, respectively). During dobutamine stress, heart rate increased from 423 +/- 50 to 603 +/- 30 beats/min (P = 0.002) and cardiac output increased from 18.5 +/- 1.9 to 32.0 +/- 4.2 mL/min (P = 0.008). CONCLUSION Parameters of cardiovascular function can be measured in mice noninvasively by radionuclide angiography using high-temporal-resolution small-animal PET. Measured values of cardiac output and stroke volume are reproducible and comparable to those obtained with MRI. The approach permits the monitoring of changes in cardiovascular function in response to pharmacologic intervention.