Silk-based biomaterials
Silk-based biomaterials
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DOI：
10.1016/s0142-9612(02)00353-8
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发表时间：
2003-02-01
期刊：
影响因子：
14
通讯作者：
Kaplan, DL
中科院分区：
文献类型：
--
作者：
Altman, GH;Diaz, F;Kaplan, DL
几个世纪以来，蚕丝一直被用作生物医学缝合材料。这些纤维独特的机械性能为许多应用提供了重要的临床修复选择。在过去的20年里，蚕丝的一些生物相容性问题被报道；然而，残留丝胶（胶状蛋白质）的污染可能是原因。最近对明确的蚕丝纤维和薄膜的研究表明，核心丝素蛋白纤维在体外和体内表现出与其他常用生物材料（例如聚乳酸和胶原蛋白）相当的生物相容性。此外，丝纤维独特的机械性能、用生长因子和粘附因子“装饰”侧链化学的多样性，以及基因定制蛋白质的能力，为探索该纤维蛋白家族在生物材料中的应用提供了额外的理由。例如，在设计组织工程支架时，这些特性特别相关，并且最近体外骨和韧带形成的结果支持了这种生物材料在未来应用中的潜在作用。迄今为止，利用蚕丝来满足生物材料和基质支架需求的研究主要集中在蚕丝上。由于蜘蛛和昆虫的丝状纤维蛋白具有多样性，一系列天然或生物工程变体有望应用于不同的临床需求。 (C) 2002 Elsevier Science Ltd. 保留所有权利。
Silk from the silkworm, Bombyx mori, has been used as biomedical suture material for centuries. The unique mechanical properties of these fibers provided important clinical repair options for many applications. During the past 20 years, some biocompatibility problems have been reported for silkworm silk; however, contamination from residual sericin (glue-like proteins) was the likely cause. More recent studies with well-defined silkworm silk fibers and films suggest that the core silk fibroin fibers exhibit comparable biocompatibility in vitro and in vivo with other commonly used biomaterials such as polylactic acid and collagen. Furthermore, the unique mechanical properties of the silk fibers, the diversity of side chain chemistries for 'decoration' with growth and adhesion factors, and the ability to genetically tailor the protein provide additional rationale for the exploration of this family of fibrous proteins for biomaterial applications. For example, in designing scaffolds for tissue engineering these properties are particularly relevant and recent results with bone and ligament formation in vitro support the potential role for this biomaterial in future applications. To date, studies with silks to address biomaterial and matrix scaffold needs have focused on silkworm silk. With the diversity of silk-like fibrous proteins from spiders and insects, a range of native or bioengineered variants can be expected for application to a diverse set of clinical needs. (C) 2002 Elsevier Science Ltd. All rights reserved.