Improved hydrolysis of alpha-tocopherol acetate emulsion and its bioaccessibility in the presence of polysaccharides and PEG2000
Improved hydrolysis of alpha-tocopherol acetate emulsion and its bioaccessibility in the presence of polysaccharides and PEG2000
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多糖和 PEG2000 存在下改善 α-生育酚醋酸酯乳液的水解及其生物可及性
DOI：
10.1016/j.colsurfa.2019.123837
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Chen Zhong Xiu
中科院分区：
文献类型：
--
作者：
Luo Xing An;Zhao Pei;Zhang Hai;Feng Shu Yi;Chen Ke Xian;Chen Zhong Xiu
膳食大分子常用于食品配方。大多数已发表的文献通常集中在某种多糖减少脂质消化上，但对非特异性的物理效应，如周围大分子的排除体积效应，可能会增强脂质水解，却很少讨论。由于多糖对乳状液包封α-生育酚乙酸酯（ve -生育酚乙酸酯，VE-A）胃肠道命运的影响尚未见报道，本研究主要研究了多糖（Ficoll400）形成的拥挤培养基对VE-A水解及其生物可及性的影响。结果表明，与稀释缓冲液相比，Ficoll400的存在使VE-A的生物可及性和转化率提高了1.6倍。酶催化VE-A水解的动力学反应速率提高了3.5倍。在合成的大分子PEG2000培养基中进一步观察了VE-A乳液的热力学转化率和水解动力学速率的提高。本研究提高了我们对大分子对VE-A/脂质乳消化的拥挤效应的理解。
Dietary macromolecules are often used for food formulation. Most of the published literature usually focuses on a certain polysaccharide reducing lipid digestion, but the non-specific physical effects such as the excluded volume effect of the surrounding macromolecules, which will possibly enhance the hydrolysis of lipids, is rarely discussed. As the influence of polysaccharides on the gastrointestinal fate of α-tocopherol acetate (VE-A) encapsulated in emulsion have not been conducted so far, in this research, we study the effect of crowded medium created by polysaccharides (Ficoll400) on the hydrolysis of VE-A and its bioaccessibility. The results show that the presence of Ficoll400 increased the bioaccessibility and conversion of VE-A 1.6 times that of a dilute buffer. An enzyme-catalyzed kinetic reaction rate of VE-A hydrolysis is increased up to 3.5 times. Both the improved thermodynamic conversion yields and kinetic rates of the hydrolysis of the VE-A emulsion was further observed in a synthetic macromolecule PEG2000 medium. This research improves our understanding of the crowding effect of macromolecules on VE-A/lipid emulsion digestion.