Small RNAs in genome rearrangement in Tetrahymena
Small RNAs in genome rearrangement in Tetrahymena
复制标题
DOI：
10.1016/j.gde.2004.01.004
复制
发表时间：
2004-04-01
影响因子：
4
通讯作者：
Gorovsky, MA
中科院分区：
文献类型：
--
作者：
Mochizuki, K;Gorovsky, MA
由RNAi相关机制产生的小RNA参与四膜虫种系微核发育成体细胞大核过程中的DNA消除。这些小RNA的特性可以解释亲本大核的一级序列如何表观遗传地控制新大核中的基因组重排，并首次证明了RNAi介导的直接改变DNA序列组织的过程。组蛋白 H3 在赖氨酸 9 上的甲基化和染色质结构域蛋白的积累（异染色质的标志）也专门发生在正在消除的序列上，并且依赖于小 RNA。这些发现有助于建立染色质生物学的新范式：真核生物中通过 RNAi 相关机制调节异染色质形成。
Small RNAs produced by an RNAi-related mechanism are involved in DNA elimination during development of the somatic macronucleus from the germline micronucleus in Tetrahymena. The properties of these small RNAs can explain how the primary sequence of the parental macronucleus epigenetically controls genome rearrangement in the new macronucleus and provide the first demonstration of an RNAi-mediated process that directly alters DNA sequence organization. Methylation of histone H3 on lysine 9 and accumulation of chromodomain proteins, hallmarks of heterochromatin, also occur specifically on sequences undergoing elimination and are dependent on the small RNAs. These findings contribute to a new paradigm of chromatin biology: regulation of heterochromatin formation by RNAi-related mechanisms in eukaryotes.