Pi sampling: a methodical and flexible approach to initial macromolecular crystallization screening.
Pi sampling: a methodical and flexible approach to initial macromolecular crystallization screening.
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DOI：
10.1107/s0907444911008754
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发表时间：
2011-05
期刊：
影响因子：
--
通讯作者：
Warne T
中科院分区：
文献类型：
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作者：
Gorrec F;Palmer CM;Lebon G;Warne T
Pi采样源自不完全析因方法，旨在最大限度地提高大分子结晶条件的多样性，并促进96条件初始筛选的制备。π抽样方法来源于大分子结晶筛设计的不完全析因方法。由此产生的“Pi屏幕”有一个模块分布的一组给定的多达36个股票的解决方案。考虑到配方中使用的化学品的性质和相应溶液的浓度，可以最大限度地产生不同的条件。Pi抽样方法已经在一个基于web的应用程序中实现，该应用程序生成屏幕配方和食谱。它特别适用于由96种不同条件组成的屏幕。可溶蛋白的结晶和完整膜蛋白样品的结晶证明了Pi采样的灵活性和效率。
Pi sampling, derived from the incomplete factorial approach, is an effort to maximize the diversity of macromolecular crystallization conditions and to facilitate the preparation of 96-condition initial screens. The Pi sampling method is derived from the incomplete factorial approach to macromolecular crystallization screen design. The resulting ‘Pi screens’ have a modular distribution of a given set of up to 36 stock solutions. Maximally diverse conditions can be produced by taking into account the properties of the chemicals used in the formulation and the concentrations of the corresponding solutions. The Pi sampling method has been implemented in a web-based application that generates screen formulations and recipes. It is particularly adapted to screens consisting of 96 different conditions. The flexibility and efficiency of Pi sampling is demonstrated by the crystallization of soluble proteins and of an integral membrane-protein sample.