CULTEX - an alternative technique for cultivation and exposure of cells of the respiratory tract to airborne pollutants at the air/liquid interface
CULTEX - an alternative technique for cultivation and exposure of cells of the respiratory tract to airborne pollutants at the air/liquid interface
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DOI：
10.1016/s0940-2993(00)80044-5
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发表时间：
2000-06-01
影响因子：
--
通讯作者：
Mohr, U
中科院分区：
文献类型：
--
作者：
Aufderheide, M;Mohr, U
由于将细胞培养物直接暴露于这些物质中存在困难，传统上对气体或颗粒化合物以及复杂混合物等空气污染物的细胞毒性评估涉及动物实验。新的培养和暴露技术提高了体外研究的效率，如一种新的实验系统CULTEX 1所示，该系统允许细胞直接暴露于空气/液体界面。在这种情况下，人支气管上皮细胞在允许间歇性培养基供应的装置中的多孔transwell膜上培养。培养基通过支持细胞的transwell膜泵入一个特殊的模块化培养单元。在某些时间间隔，培养基被完全去除，并且细胞可以保持并暴露在空气/液体界面处，直到下一次培养基供应而不丧失活力。与传统的浸没培养条件相比，使用新的脉冲浸没技术，细胞在transwell膜上生长。由于直接暴露于空气污染物，对细胞活力没有有害影响。因此，这些新的培养和接触技术的引入为可吸入可溶性和惰性物质以及复杂混合物的毒理学评价提供了新的测试策略。
The assessment of cytotoxicity of air contaminants such as gaseous or particulate compounds and complex mixtures has traditionally involved animal experiments, due to the difficulties in exposing cell cultures directly to these substances.New cultivation and exposure techniques enhance the efficiency of in vitro studies, as demonstrated by a new experimental system called CULTEX1 which allows direct exposure of cells at the air/liquid interface. In this case, human bronchial epithelial cells are cultivated on porous transwell membranes in a device allowing intermittent medium supply. The medium is pumped into a special modular culture unit through the transwell membrane supporting the cells. At certain time intervals, the medium is completely removed and the cells can be maintained and exposed at the air/liquid interface until the next medium supply without loss of viability. In comparison to conventional submersed culture conditions, the cells have been grown on transwell membranes using the new pulse submersion technique. There are no deleterious effects on cell viability due to the direct exposure to airborne pollutants. Thus, the introduction of these new cultivation and exposure techniques offers new testing strategies for the toxicological evaluation of inhalable soluble and inert substances as well as complex mixtures.