Acute metabolic acidosis augments collecting duct acidification rate in the rat.
Acute metabolic acidosis augments collecting duct acidification rate in the rat.
复制标题
急性代谢性酸中毒会增加大鼠集合管酸化率。
DOI：
10.1152/ajprenal.1981.241.6.f669
复制
发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Alexander,EA
中科院分区：
文献类型：
--
作者：
Graber,ML;Bengele,HH;Mroz,E;Lechene,C;Alexander,EA
通常认为，远端酸化的至少一部分沿着集合管发生。然而，完整和直接的评价酸化沿着这段肾单位是不可用的。这些实验的目的是量化沿着内髓集合管（IMCD）的净酸化率，并测量急性HCl酸中毒的影响。在13只对照大鼠中（动脉pH，7.39 +/- 0.01; PCO 2，39 +/- 1 mmHg）和11只HCl输注大鼠（动脉pH 7.18 ± 0.01;二氧化碳分压，40 +/- 1 mmHg）我们通过改良的微导管技术获得了4到8个IMCD样本，该技术还允许测量原位和体外pH值。肾小管液pH值沿着IMCD降低在两组中，并且在酸注入大鼠中酸性更强0.2-0.4 pH单位。在两组中均观察到Bicarbine重吸收，因为对照组大鼠沿着IMCD的递送从205 +/- 127 nmol/min降至26 +/- 6 nmol/min，酸中毒组从219 +/- 118 nmol/min降至17 +/- 4 nmol/min。在酸中毒大鼠中，氨向IMCD的输送和沿着IMCD的添加显著更大-对照组从193 +/- 59到462 +/- 53 nmol/min，酸中毒大鼠从887 +/- 126到1，396 +/- 90 nmol/min。未观察到总磷酸盐或滴定磷酸盐的显著变化。净酸添加沿着IMCD是在酸中毒大鼠的两倍以上，450与970 nmol/min。我们的研究结果表明，显着的净酸添加发生沿着IMCD和酸中毒过程中，这种酸化率增加。
It is generally assumed that at least part of distal acidification occurs along the collecting duct. Complete and direct evaluation of acidification along this nephron segment is unavailable, however. The purpose of these experiments was to quantify the net acidification rate along the inner medullary collecting duct (IMCD) and to measure the effect of acute HCl acidosis. In 13 control rats (arterial pH, 7.39 +/- 0.01; PCO2, 39 +/- 1 mmHg) and 11 HCl-infused rats (arterial pH 7.18 +/- 0.01; PCO2, 40 +/- 1 mmHg) we obtained four to eight IMCD samples by a modified microcatheterization technique that also permitted measurement of in situ and in vitro pH. Tubular fluid pH decreased along the IMCD in both groups and was more acidic by 0.2–0.4 pH units in the acid-infused rats. Bicarbonate reabsorption was noted in both groups as delivery along the IMCD decreased from 205 +/- 127 to 26 +/- 6 nmol/min in control rats and from 219 +/- 118 to 17 +/- 4 nmol/min in the acidotic group. Ammonia delivery to and addition along the IMCD was significantly greater in the acidotic rats--from 193 +/-- 59 to 462 +/- 53 nmol/min in control and from 887 +/- 126 to 1,396 +/- 90 nmol/min in acidotic rats. No significant change in total or titrated phosphate was seen. Net acid addition along the IMCD was over twice as great in acidotic rats, 450 vs. 970 nmol/min. Our results indicate that significant net acid addition occurs along the IMCD and that during acidosis this acidification rate increases.
白内障囊外摘除术和人工晶状体植入术中手术显微镜引起的光诱发黄斑病变。
DOI：
--
发表时间：
1983
期刊：
Ophthalmology (Rochester, Minn.)
影响因子：
--
作者：
H. McDonald;A. Irvine
通讯作者：
A. Irvine