Colonic mucin: methods of measuring mucus thickness
Colonic mucin: methods of measuring mucus thickness
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DOI：
10.1079/pns2002205
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发表时间：
2003-02-01
影响因子：
7
通讯作者：
Pearson, JP
中科院分区：
文献类型：
--
作者：
Strugala, V;Allen, A;Pearson, JP
粘液是由胃肠道分泌的一种不溶于水的凝胶。它作为粘附在胃、小肠和结肠上皮表面的保护凝胶层存在。黏液凝胶由1- 10% （w/v）的黏蛋白糖蛋白在血浆样液体中组成。由于粘液凝胶主要是水，标准的组织学技术使粘液脱水，使功能屏障的可视化变得困难。已经开发了专门的技术来实现完整黏液层的可视化。将描述一种简单的组织学方法，使用快速冷冻组织，用低温恒温器切片，并在粘液保存条件下用改良的周期性酸-希夫斯/阿利新蓝染色。第二个强大的体内动物模型描述，使粘液分泌随时间的测量。使用这两种方法，可以确定结肠中正常黏液层的特征，并确定疾病和饮食干预对其的影响，特别是膳食纤维的影响，并提供了纤维缺乏导致结肠粘膜脆弱的证据。
Mucus is a water-insoluble gel secreted by the gastrointestinal tract. It exists as a protective gel layer adherent to the epithelial surface of the stomach, small intestine and colon. The mucus gel is composed of 1-10 % (w/v) mucin glycoprotein in a plasma-like fluid. Since the mucus gel is predominantly water, standard histological techniques dehydrate the mucus, making visualisation of the functional barrier difficult. Specialist techniques have been developed to enable visualisation of the intact mucus layer. A simple histological method using snap-frozen tissue, sectioned with a cryostat and stained with modified periodic acid-Schiffs/Alcian blue in mucus-preserving conditions will be described. A second powerful in vivo animal model is described which enables measurement of mucus secretion over time. The use of these two methods has allowed the characterisation of the normal mucus layer in the colon and the determination of how it is affected by disease and dietary intervention, in particular the effect of dietary fibre, and evidence that fibre deficiency results in colonic mucosal fragility is presented.