Indoor air particles and bioaerosols before and after renovation of moisture-damaged buildings:: The effect on biological activity and microbial flora
Indoor air particles and bioaerosols before and after renovation of moisture-damaged buildings:: The effect on biological activity and microbial flora
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DOI：
10.1016/j.envres.2008.02.008
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发表时间：
2008-07-01
影响因子：
8.3
通讯作者：
Nevalainen, Aino
中科院分区：
文献类型：
--
作者：
Huttunen, Kati;Rintala, Helena;Nevalainen, Aino
许多与建筑物有关的健康问题与建筑物内的潮湿损害和霉菌生长相吻合。消除它们被认为可以改善室内空气质量。本研究的目的是通过分析过滤样品的微生物植物群和免疫毒理学活性来跟踪两个单独建筑物的修复成功。我们比较了从潮湿损坏的建筑物装修前后的室内空气中收集的样品的结果，以及从匹配的参考建筑物和室外空气中收集的结果。通过麦角甾醇含量分析和定量聚合酶链反应（QPCR）测定真菌植物群组成，研究了样品的微生物特性。此外，用光学粒子计数器（OPC）监测粒子的浓度。通过将小鼠巨噬细胞（RAW264.7）暴露于从过滤器中提取的颗粒悬浮液24 h，并通过酶联免疫测定法（ELISA）测量暴露细胞的活力（MTT试验）和细胞培养基中炎症介质（一氧化氮、IL-6和TNF α）的产生，检测收集颗粒样品的免疫毒理学活性。结果表明，对于位置I的装修降低了从受损建筑物收集的颗粒物的免疫毒理学活性，而在从参考建筑物收集的相应样品中没有检测到差异。有趣的是，在真菌的浓度中仅观察到轻微的差异。在位置2中，翻新后真菌浓度降低，但未检测到对免疫毒理学反应的影响。在这种情况下，对室内空气样品的免疫毒理学反应与室外空气样品引起的免疫毒理学反应几乎相同。这表明，补救措施对室内空气质量的影响可能不一定容易用微生物或毒理学参数来衡量。这可能与不同的霉菌和潮湿损坏的建筑物中有害物质的不同谱有关。(C)2008年爱思唯尔公司All rights reserved.
Many building-related health problems coincide with moisture damage and mold growth within a building. Their elimination is assumed to improve indoor air quality. The aim of this study was to follow the success of remediation in two individual buildings by analyzing the microbial flora and immunotoxicological activity of filter samples. We compare results from samples collected from indoor air in the moisture-damaged buildings before and after renovation and results from matched reference buildings and outdoor air. The microbial characteristics of the samples were studied by analyzing ergosterol content and determining the composition of fungal flora with quantitative polymerase chain reaction (QPCR). In addition, the concentrations of particles were monitored with optical particle counter (OPC). The immunotoxicological activity of collected particle samples was tested by exposing mouse macrophages (RAW264.7) for 24h to particle suspension extracted from the filters, and measuring the viability of the exposed cells (MTT-test) and production of inflammatory mediators (nitric oxide, IL-6 and TNF alpha) in cell culture medium by enzyme-linked immunoassay (ELISA). The results show that for Location I the renovation decreased the immunotoxicological activity of the particles collected from damaged building, whereas no difference was detected in the corresponding samples collected from the reference building. Interestingly, only slight differences were seen in the concentration of fungi. In the Location 2, a decrease was seen in the concentration of fungi after the renovation, whereas no effect on the immunotoxicological responses was detected. In this case, the immunotoxicological responses to the indoor air samples were almost identical to those caused by the samples from outdoor air. This indicates that the effects of remediation on the indoor air quality may not necessarily be readily measurable either with microbial or toxicological parameters. This may be associated with different spectrum of harmful agents in different mold and moisture-damaged buildings. (C) 2008 Elsevier Inc. All rights reserved.