Analysis of cell lineage relationships in taste buds
Analysis of cell lineage relationships in taste buds
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DOI：
10.1523/jneurosci.22-11-04522.2002
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发表时间：
2002-06-01
影响因子：
5.3
通讯作者：
Finger, TE
中科院分区：
文献类型：
--
作者：
Stone, LM;Tan, SS;Finger, TE
味蕾是一个异质的细胞群体，表现出不同的形态和生化特征。由于味蕾来自多个祖细胞，不同类型的味觉细胞可能代表不同的谱系。本研究旨在确定以下内容：（1）有多少祖细胞对味蕾有贡献，以及（2）特定的味觉细胞亚群是否与一组有限的祖细胞谱系相关。使用H253 X失活嵌合体小鼠味蕾的细胞谱系分析来解决这些问题。在lacZ转基因的随机X失活后，半合子雌性小鼠的舌头显示上皮细胞的离散斑块，其为β-半乳糖苷酶（β-gal）阳性或β-gal阴性。通过分析位于阳性和阴性上皮斑块之间的边界处的味蕾中两种不同染色的细胞群体的比例，我们可以确定可能有助于味蕾形成的祖细胞的最小数量。仅含有6-12%标记细胞的味蕾的存在表明至少8个祖细胞贡献于平均55个细胞的味蕾，假设祖细胞对细胞群的贡献相等。在这样的混合味蕾中的胡萝卜素-IR味细胞的细胞谱系分析表明，该亚群可能仅来自一个至两个祖细胞，并且通常通过谱系相关。因此，味蕾中的至少一些细胞类型代表不同的细胞谱系，而不仅仅是细胞从年轻状态发展到成熟状态的表型阶段。
Taste buds are a heterogeneous population of cells exhibiting diverse morphological and biochemical characteristics. Because taste buds arise from multiple progenitors, the different types of taste cells may represent distinct lineages. The present study was undertaken to determine the following: (1) how many progenitors contribute to a taste bud, and (2) whether the specific subpopulation of serotonin-immunoreactive (IR) taste cells are related by lineage to a restricted set of progenitor cells. These questions were addressed using cell lineage analysis of taste buds from H253 X-inactivation mosaic mice. After random X-inactivation of the lacZ transgene, the tongue of hemizygous female mice displays discrete patches of epithelial cells, which are either beta-galactosidase (beta-gal) positive or beta-gal negative. By analyzing the proportion of the two differently stained cell populations in taste buds located at the boundary between positive and negative epithelial patches, we can determine the minimum number of progenitors that may contribute to the formation of a taste bud. The presence of taste buds containing only 6-12% labeled cells indicates that at least eight progenitors contribute to an average taste bud of 55 cells, assuming progenitors contribute equally to the cell population. Cell lineage analysis of serotonin-IR taste cells in such mixed taste buds suggests that this subpopulation likely arises from only one to two progenitors and often is related by lineage. Thus, at least some of the cell types in a taste bud represent distinct lineages of cells and are not merely phenotypic stages as a cell progresses from a young to a mature state.