Heterogeneous distribution of doublecortin-expressing cells surrounding the rostral migratory stream in the juvenile mouse
Heterogeneous distribution of doublecortin-expressing cells surrounding the rostral migratory stream in the juvenile mouse
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DOI：
10.1002/cne.24521
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发表时间：
2018-11-01
影响因子：
2.5
通讯作者：
Nemoto, Tomomi
中科院分区：
文献类型：
--
作者：
Aoyagi, Yuka;Hibi, Terumasa;Nemoto, Tomomi
在出生后的哺乳动物脑中，脑室-脑室下区的神经干细胞继续产生表达双皮质素（Dcx）的未成熟神经元。在整个生命过程中，这些未成熟的神经元通过吻侧迁移流（RMS）迁移到嗅球。在这项研究中，我们调查了这些假定的未成熟神经元的分布，使用增强的绿色荧光蛋白（EGFP）的表达在周围地区的RMS的青少年Dcx-EGFP小鼠。通过结合使用光学透明试剂（2，2 '-硫代二乙醇溶液）和双光子显微镜，我们三维可视化了整个RMS及其周围的EGFP阳性细胞。所得到的宽视场和高清晰度图像沿着与本研究中开发的计算图像处理方法一起用于全面确定EGFP阳性细胞的位置。我们的研究结果显示，EGFP阳性细胞不均匀地分布在RMS周围的区域。此外，这些细胞的主导过程的方向模式，显示迁移的未成熟神经元的形态，根据其位置而不同。这些新的结果提供了高度精确的形态学信息的未成熟的神经元，并建议，一部分未成熟的神经元可能脱离RMS和迁移在各个方向。
In the postnatal mammalian brain, neural stem cells of the ventricular-subventricular zone continue to generate doublecortin (Dcx)-expressing immature neurons. Throughout life, these immature neurons migrate to the olfactory bulb through the rostral migratory stream (RMS). In this study, we investigated the distribution of these putative immature neurons using enhanced green fluorescent protein (EGFP) expression in the area surrounding the RMS of the juvenile Dcx-EGFP mice. Through the combined use of an optical clearing reagent (a 2,2 '-thiodiethanol solution) and two-photon microscopy, we visualized three-dimensionally the EGFP-positive cells in the entire RMS and its surroundings. The resulting wide-field and high-definition images along with computational image processing methods developed in this study were used to comprehensively determine the position of the EGFP-positive cells. Our findings revealed that the EGFP-positive cells were heterogeneously distributed in the area surrounding the RMS. In addition, the orientation patterns of the leading process of these cells, which displayed the morphology of migrating immature neurons, differed depending on their location. These novel results provide highly precise morphological information for immature neurons and suggest that a portion of immature neurons may be detached from the RMS and migrate in various directions.