RNA-Seq Reveals OTA-Related Gene Transcriptional Changes in Aspergillus carbonarius.
RNA-Seq Reveals OTA-Related Gene Transcriptional Changes in Aspergillus carbonarius.
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DOI：
10.1371/journal.pone.0147089
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Faretra F
中科院分区：
文献类型：
--
作者：
Gerin D;De Miccolis Angelini RM;Pollastro S;Faretra F
赭曲霉毒素A（OTA）是一种对动物和人类有害的真菌毒素。碳曲霉是造成葡萄及其衍生产品OTA污染的主要原因。4A的基因转录谱分析。通过RNA-Seq分析对carbonarius菌株进行了筛选，以研究与OTA产生相关的转录组变化。通过比较OTA诱导（OTAI）与非诱导（OTAN）培养条件，总共3，705个差异表达基因（DEG）（倍数变化>| 2| FDR ≤ 0.05）。参与初级代谢过程的几个基因，特别是关于碳水化合物和氨基酸代谢，次级代谢过程，运输，应激反应和孢子形成的上调OTAI条件在所有分析的采样时间（4，6和8 DAI）或从6 DAI。高度上调的DEG编码参与次生代谢物、氧化还原酶、转运蛋白和转录因子的生物合成的酶，检查其潜在参与OTA生物合成和相关代谢途径。通过RT-qPCR验证编码聚酮酶（PKs）、非核糖体肽合成酶（Nrps）和氯过氧化物酶（CPO）的基因的差异表达。在SM生物合成的共调控基因簇中，在A. carbonarius基因组。
Ochratoxin A (OTA) is a mycotoxin harmful for animals and humans. Aspergillus carbonarius is the main responsible for OTA contamination of grapes and derived products. Gene transcriptional profiling of 4 A. carbonarius strains was carried out by RNA-Seq analysis to study transcriptome changes associated with OTA production. By comparing OTA inducing (OTAI) vs. non-inducing (OTAN) cultural conditions, a total of 3,705 differentially expressed genes (DEGs) (fold change > |2| and FDR ≤ 0.05) were identified. Several genes involved in primary metabolic processes, with particular regard to carbohydrate and amino acid metabolisms, secondary metabolic processes, transport, response to stress and sporulation were up-regulated by OTAI conditions at all the analysed sampling times (4, 6 and 8 DAI) or starting from 6 DAI. Highly up-regulated DEGs encoding enzymes involved in biosynthesis of secondary metabolites, oxidoreductases, transporters and transcription factors were examined for their potential involvement in OTA biosynthesis and related metabolic pathways. Differential expression of genes encoding polyketide synthases (pks), non-ribosomal peptide synthetases (nrps) and chloroperoxidase (cpo) was validated by RT-qPCR. Among clusters of co-regulated genes involved in SM biosynthesis, one putative OTA-gene cluster, including both pks and nrps genes, was detected in the A. carbonarius genome.