Effect of hemolysate on calcium inhibition of the (Na+ + K+)-ATPase of human red blood cells.
Effect of hemolysate on calcium inhibition of the (Na+ + K+)-ATPase of human red blood cells.
复制标题
溶血产物对人红细胞 (Na K)-ATP 酶钙抑制的影响。
DOI：
10.1016/0006-291x(83)91395-5
复制
发表时间：
1983
影响因子：
3.1
通讯作者：
M. Marcovitz
中科院分区：
文献类型：
--
作者：
D. Yingst;M. Marcovitz
人红细胞膜Na++ K+-ATP酶对Ca 2+抑制的敏感性可因加入稀释的溶血红细胞胞浆而显著增加。与体内浓度相比，当将1：60稀释的溶血产物加入测定混合物中时，引起（Na++ K+）-ATP酶50%抑制的Ca 2+浓度从不存在溶血产物时大于50 μ M游离Ca 2+变为小于10 μM游离Ca 2+。煮沸溶血产物破坏了其增加（Na++ K+）-ATP酶对Ca 2+敏感性的能力。在EDTA存在下从膜中提取的蛋白质在Amicon PM 30膜上浓缩，以剂量依赖的方式增加（Na++ K+）-ATP酶对Ca 2+的敏感性，在10 μM游离Ca 2+下，在最高浓度的测试提取物对（Na++ K+）-ATP酶的抑制超过80%。这种膜提取物中的活性因子是依赖于Ca 2+的，因为它在无Ca 2+时对（Na++ K+）-ATP酶没有影响。胰蛋白酶消化破坏了该蛋白提取物增加（Na++ K+）-ATP酶对Ca 2+敏感性的能力。
The sensitivity of the (Na++ K+-ATPase in human red cell membranes to inhibition by Ca 2+ is markedly increased by the addition of diluted cytoplasm from hemolyzed human red blood cells. The concentration of Ca 2+ causing 50% inhibition of the (Na++ K+)-ATPase is shifted from greater than 50 μM free Ca 2+ in the absence of hemolysate to less than 10 μM free Ca 2+ when hemolysate diluted 1: 60 compared to in vivo concentrations is added to the assay mixture. Boiling the hemolysate destroys its ability to increase the sensitivity of the (Na++ K+)-ATPase to Ca 2+. Proteins extracted from the membrane in the presence of EDTA and concentrated on an Amicon PM 30 membrane increased the sensitivity of the (Na++ K+)-ATPase to Ca 2+ in a dose-dependent fashion, causing over 80% inhibition of the (Na++ K+)-ATPase at 10 μM free Ca 2+ at the highest concentration of the extract tested. The active factor in this membrane extract is Ca 2+-dependent, because it had no effect on the (Na++ K+)-ATPase in the absence of Ca 2+. Trypsin digestion prior to the assay destroyed the ability of this protein extract to increase the sensitivity of the (Na++ K+)-ATPase to Ca 2+.