Chronic exposure to an activator of protein kinase C mimics early effects of NGF in chromaffin cells.
Chronic exposure to an activator of protein kinase C mimics early effects of NGF in chromaffin cells.
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长期接触蛋白激酶 C 激活剂可模拟嗜铬细胞中 NGF 的早期作用。
DOI：
10.1016/0012-1606(91)90256-3
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发表时间：
1991
影响因子：
2.7
通讯作者：
Claude,P
中科院分区：
文献类型：
--
作者：
Herman,MA;Schulz,CA;Claude,P
肾上腺嗜铬细胞在体外对神经生长因子（NGF）的反应是表达神经元的特征，并在2 - 4周的时间内转分化为有丝分裂后的交感神经元。佛波醇肉豆蔻酸酯乙酸酯（PMA）是一种有效的激活剂蛋白激酶C（PKC）;长期暴露于PMA模拟神经生长因子的初始行动，促进神经突起的生长和增加掺入的[3 H]胸苷在原代培养的肾上腺嗜铬细胞从年轻的大鼠。PMA和NGF影响相同的细胞群，甚至单个神经突。这些效果是特定的活性佛波醇酯，并不导致在文化中的NGF或FGF的释放。在神经生长因子的情况下，糖皮质激素抑制的影响。PKC抑制剂staurosporine以剂量依赖性方式抑制PMA以及NGF的作用。这些结果表明，PKC活性的调制是重要的神经生长因子的神经轴突和增殖的影响，至少在最初的一段时间约1周。
Adrenal chromaffin cells respond to nerve growth factor (NGF)in vitroby expressing neuronal characteristics and, over a period of 2 to 4 weeks, transdifferentiating into postmitotic sympathetic neurons. Phorbol myristate acetate (PMA) is a potent activator of protein kinase C (PKC); chronic exposure to PMA mimics the initial actions of NGF by promoting the outgrowth of neurites and increasing the incorporation of [3H]thymidine in primary cultures of adrenal chromaffin cells from young rats. PMA and NGF affect the same populations of cells and even individual neurites. These effects are specific for active phorbol ester and do not result from the release of NGF or FGF in the cultures. As in the case of NGF, the effects are inhibited by glucocorticoids. The PKC inhibitor staurosporine inhibits the effects of PMA, as well as those of NGF, in a dose-dependent manner. These results suggest that a modulation in activity of PKC is important in the neuritogenic and proliferative effects of NGF, at least for an initial period of approximately 1 week.