Quantitative Shotgun Proteomics Analysis of Rice Anther Proteins after Exposure to High Temperature.
Quantitative Shotgun Proteomics Analysis of Rice Anther Proteins after Exposure to High Temperature.
复制标题
DOI：
10.1155/2015/238704
复制
发表时间：
2015
影响因子：
2.9
通讯作者：
Lee J
中科院分区：
文献类型：
--
作者：
Kim M;Kim H;Lee W;Lee Y;Kwon SW;Lee J
在水稻中，对高温胁迫最敏感的发育阶段是开花，并且在该阶段暴露可导致小穗不育，从而导致显著的产量损失。在这项研究中，蛋白质表达模式的水稻花药滇西4号，耐高温粳稻品种，暴露于高温和那些生长在韩国的自然田间条件下的样品进行了比较。对三个重复的对照和高温处理的样品的鸟枪蛋白质组学分析鉴定了3，266个非冗余的水稻花药蛋白（错误发现率< 0.01）。我们发现，高水平的ATP合酶，cupin结构域的蛋白质，花粉过敏原蛋白存在于水稻花药。对1，944个可重复表达的蛋白质进行比较分析，确定了139个差异表达的蛋白质，其中95个在高温条件下增加，44个减少。热激、DnaK家族和分子伴侣蛋白的表达量显著增加，表明滇系4号的高温耐受性是通过稳定花粉细胞中的蛋白质来实现的。热处理后海藻糖合酶也高度增加，这表明海藻糖可能在防止蛋白质通过干燥变性中起作用。
In rice, the stage of development most sensitive to high temperature stress is flowering, and exposure at this stage can result in spikelet sterility, thereby leading to significant yield losses. In this study, protein expression patterns of rice anthers from Dianxi4, a high temperature tolerant Japonica rice variety, were compared between samples exposed to high temperature and those grown in natural field conditions in Korea. Shotgun proteomics analysis of three replicate control and high-temperature-treated samples identified 3,266 nonredundant rice anther proteins (false discovery rate < 0.01). We found that high levels of ATP synthase, cupin domain-containing proteins, and pollen allergen proteins were present in rice anthers. Comparative analyses of 1,944 reproducibly expressed proteins identified 139 differentially expressed proteins, with 95 increased and 44 decreased in response to high temperature conditions. Heat shock, DnaK family, and chaperone proteins showed highly increased expression, suggesting that the high temperature tolerance of Dianxi4 is achieved by stabilization of proteins in pollen cells. Trehalose synthase was also highly increased after heat treatment, suggesting a possible role for trehalose in preventing protein denaturation through desiccation.