Bioimaging application of highly luminescent silica-coated ZnO-nanoparticle quantum dots with biotin
Bioimaging application of highly luminescent silica-coated ZnO-nanoparticle quantum dots with biotin
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DOI：
10.1016/j.jcis.2013.02.047
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发表时间：
2013-06-01
影响因子：
9.9
通讯作者：
Muto, Hiroyuki
中科院分区：
文献类型：
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作者：
Matsuyama, Kiyoshi;Ihsan, Neil;Muto, Hiroyuki
我们合成了zno纳米粒子量子点（QDs）作为生物荧光探针。采用溶胶-凝胶法制备了分散在水介质中的高发光二氧化硅包覆zno纳米粒子量子点。采用二氧化硅包覆ZnO纳米粒子量子点，提高ZnO纳米粒子的水稳定性。首先通过添加3-氨基丙基三甲氧基硅烷在二氧化硅包覆的zno纳米粒子量子点表面引入NH2基团，然后通过与磺基琥珀酰氨基-6-（生物素-氨基）己酸酯（磺基- nhs - lc -bioton）进行生物素化。我们证明了通过生物素与亲和素的选择性相互作用，用二氧化硅包覆的zno纳米粒子量子点标记了亲和素固定化琼脂糖珠，在紫外光激发下提供了荧光图像。此外，还尝试了将二氧化硅包被的含生物素的zno纳米粒子量子点用于细胞标记，并实现了二氧化硅包被的含生物素的zno纳米粒子量子点附着在神经细胞和肌动蛋白细丝上。(C) 2013爱思唯尔公司版权所有。
We synthesized ZnO-nanoparticle quantum dots (QDs) as a fluorescent probe for biological applications. Highly luminescent silica-coated ZnO-nanoparticle QDs dispersed in an aqueous medium were synthesized using the sol-gel process. The ZnO-nanoparticle QDs were coated with silica to improve the water stability of the ZnO nanoparticles. NH2 groups were introduced on the surface of the silica-coated ZnO-nanoparticle QDs first by the addition of 3-aminopropyltrimethoxysilane and then by biotinylation with sulfosuccinimidyl-6-(biotin-amido) hexanoate (sulfo-NHS-LC-bioton). We demonstrated that avidin-immobilized agarose beads were tagged by the silica-coated ZnO-nanoparticle QDs with biotin by the selective avidin-biotin interaction, furnishing a fluorescent image upon excitation with UV light. Furthermore, use of the silica-coated ZnO-nanoparticle QDs with biotin in cell-labeling applications was attempted, and attachment of the silica-coated ZnO-nanoparticle QDs with biotin to nerve cells and actin filaments was achieved. (C) 2013 Elsevier Inc. All rights reserved.