THE FERTILIZING CAPACITY OF SEA URCHIN SPERM RAPIDLY DECREASES AFTER INDUCTION OF THE ACROSOME REACTION *
THE FERTILIZING CAPACITY OF SEA URCHIN SPERM RAPIDLY DECREASES AFTER INDUCTION OF THE ACROSOME REACTION *
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海胆精子的受精能力在顶体反应诱导后迅速下降*
DOI：
10.1111/j.1440-169x.1979.00061.x
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发表时间：
1979
期刊：
影响因子：
4.6
通讯作者：
V. Vacquier
中科院分区：
文献类型：
--
作者：
V. Vacquier
将静止的、无鞭毛的精子添加到紫球虫的酸脱胶卵子中，受精率为11%。添加可溶性卵胶可使受精率提高到90.5%。暴露在蛋冻中的精子中，超过50%的人经历了顶体反应，而在没有蛋冻的情况下，这一比例仅为3%-5%。在无鞭毛的精子中加入卵冻以诱导顶体反应，然后在不同时间加入脱胶的卵子。精子受精能力下降符合一级动力学规律，顶体反应诱导后23s，受精能力下降50%。完整的、可移动的精子与甲醛固定的卵子结合，最大结合发生在添加精子后40秒。40秒后，精子开始从固定的卵子上分离，240秒后，没有一个精子附着在一起。精子从固定的卵子上脱落与顶体反应后受精能力的丧失显示出密切的时间相关性。
When immotile, flagella‐less sperm were added to acid‐dejellied eggs of Strongylocentrotus purpuratus 11% of the eggs fertilized. Addition of soluble egg jelly increased the percentage fertilization to 90.5. Over 50% of the sperm exposed to egg jelly had undergone the acrosome reaction compared to only 3–5% in the absence of jelly. Egg jelly was added to flagella‐less sperm to induce the acrosome reaction and dejellied eggs added at various times thereafter. The fertilizing capacity of the sperm decreased with first order kinetics with 50% loss by 23 sec after induction of the acrosome reaction. Intact, motile sperm bind to formaldehyde‐fixed eggs with maximum binding occurring 40 sec after sperm addition. After 40 sec the sperm begin to detach from the fixed eggs and by 240 sec none remain attached. Sperm detachment from fixed eggs and loss of fertilizing capacity after the acrosome reaction show a close temporal correlation.