Weight Increase Is Associated with Skeletal Muscle Immunostaining for Advanced Glycation End Products, Receptor for Advanced Glycation End Products, and Oxidation Injury
Weight Increase Is Associated with Skeletal Muscle Immunostaining for Advanced Glycation End Products, Receptor for Advanced Glycation End Products, and Oxidation Injury
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DOI：
10.1089/rej.2008.0786
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发表时间：
2008-12-01
影响因子：
2.6
通讯作者：
Bunout, Daniel
中科院分区：
文献类型：
--
作者：
de la Maza, Maria Pia;Uribarri, Jaime;Bunout, Daniel
背景：在糖尿病患者和非糖尿病受试者中，晚期糖基化终末产物（AGEs）的组织蓄积与衰老有关。目的：本研究的目的是评估免疫染色的AGEs，特别是羧甲基赖氨酸（CML）和受体AGEs（CML），在不同的年龄和体重stability.Methodology的健康男性受试者的肌肉组织：肌肉组织中获得疝手术期间的中年男性报告体重维持（WM，n=10）或体重增加（WG，n=7），也在4个老年男性。结果：CML、CML、CML染色强度与年龄、体重变化呈高度相关（r=0.84），CML、CML染色强度与年龄、体重变化呈高度相关（r=0.84）。肌肉AGEs增生与肌肉表达的氧化损伤（8-羟基-脱氧鸟苷和4-羟基-2-壬烯醛）和炎症标记物（肿瘤坏死因子-a）的统计学相关。讨论：增加骨骼肌AGEs/β-内酰胺酶和炎症和氧化损伤的标记物与体重增加和老年提示AGEs在体重增加和衰老的肌肉减少症的致病作用。
Background: Tissue accumulation of advanced glycation end products (AGEs) is associated with ageing, both in diabetics and nondiabetic subjects. Aim: The purpose of this study was to assess immunostaining for AGEs, specifically carboxymethyl-lysine (CML) and receptor for AGEs (RAGE), in muscle tissue of healthy male subjects differing in age and weight stability.Methodology: Muscle tissue was obtained during hernia surgery in middle-aged men reporting weight maintenance (WM, n=10) or weight gain (WG, n=7), and also in 4 elderly men. Tissue immunostaining for CML and RAGE was performed.Results: Intensity of CML, and RAGE staining were highly correlated (r=0.84) and also significantly associated with weight change and age. Muscle AGEs accretion was statistically associated with muscle expression of oxidative injury (8-hydroxy-deoxyguanosine and 4-hydroxy-2-nonenal) and inflammatory markers (tumor necrosis factor-a).Discussion: The increase of skeletal muscle AGEs/RAGE and markers of inflammation and oxidative injury in association with weight gain and old age suggest a pathogenic role of AGEs in weight gain and in sarcopenia of aging.