Inactivation of hippocampus or caudate nucleus with lidocaine differentially affects expression of place and response learning
Inactivation of hippocampus or caudate nucleus with lidocaine differentially affects expression of place and response learning
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DOI：
10.1006/nlme.1996.0007
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发表时间：
1996-01-01
影响因子：
2.7
通讯作者：
McGaugh, JL
中科院分区：
文献类型：
--
作者：
Packard, MG;McGaugh, JL
通过注入利多卡因使双侧脑内这些区域失活来检查海马体和尾状核的就地受累和反应学习。训练大鼠从相同的起始箱(每天4次，共14天)在交叉迷宫中接近持续放置诱饵的手臂。在第8天和第16天进行单探针实验，将大鼠放在训练中使用的起始箱对面的起始箱中，允许大鼠接近迷宫臂。在探针试验前三分钟，大鼠接受了双侧注射生理盐水或2%利多卡因溶液(以产生神经失活)到背侧海马区或背侧尾状核。在探测实验中，进入有诱饵迷宫手臂的大鼠(即在训练期间接近食物所在的地方)被指定为地点学习者，进入无诱饵迷宫手臂的大鼠(即做出与训练时相同的转身反应)被指定为反应学习者。生理盐水处理的大鼠在第8天的探测试验中表现出位置学习，在第16天的探测试验中表现出反应学习，这表明随着训练的延长，控制行为的学习机制发生了变化。在第8天的探测试验中，向海马区注射利多卡因的大鼠没有表现出对位置或反应学习的偏好，但在第16天的探测试验中显示出反应学习，这表明在海马体失活后，位置学习被阻断。在第8天和第16天的探测试验中，向尾状核注射利多卡因的大鼠都显示出位置学习，这表明尾状核失活后反应学习受阻。结果表明：(1)海马和尾状核分别选择性地调节位置学习和反应学习的表达；(2)在视觉线索迷宫外环境中，海马依赖的位置学习比尾状核依赖的反应学习获得得更快；(3)当动物通过延长训练转向尾核依赖的反应学习时，基于海马体的位置表征保持不变。(C)1996年学术出版社。
Involvement of the hippocampus and caudate nucleus in place and response learning was examined by functionally inactivating these brain regions bilaterally with infusions of lidocaine. Rats were trained to approach a consistently baited arm in a cross-maze from the same start box (four trials/day/14 total days). On Days 8 and 16 a single probe trial was given, in which rats were placed in the start box opposite that used in training and allowed to approach a maze arm. Three minutes prior to the probe trial, rats received bilateral injections of either saline or a 2% lidocaine solution (in order to produce neural inactivation) into either the dorsal hippocampus or dorsolateral caudate nucleus. On the probe trials, rats which entered the baited maze arm (i.e., approached the place where food was located during training) were designated place learners, and rats which entered the unbaited maze arm (i.e., made the same turning response as during training) were designated response learners. Saline-treated rats displayed place learning on the Day 8 probe trial and response learning on the Day 16 probe trial, indicating that with extended training there is a shift in learning mechanisms controlling behavior. Rats given lidocaine injections into the hippocampus showed no preference for place or response learning on the Day 8 probe trial, but displayed response learning on the Day 16 probe trial, indicating a blockade of place learning following inactivation of the hippocampus. Rats given lidocaine injections into the caudate nucleus displayed place learning on both the Day 8 and the Day 16 probe trials, indicating a blockade of response learning following inactivation of the caudate nucleus. The findings indicate: (1) the hippocampus and caudate nucleus selectively mediate expression of place and response learning, respectively (2), in a visually cued extramaze environment, hippocampal-dependent place learning is acquired faster than caudate-dependent response learning, and (3) when animals shift to caudate-dependent response learning with extended training, the hippocampal-based place representation remains intact. (C) 1996 Academic Press, Inc.