New aspects of gamete transport, fertilization, and embryonic development in the oviduct gained by means of live cell imaging
New aspects of gamete transport, fertilization, and embryonic development in the oviduct gained by means of live cell imaging
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DOI：
10.1016/j.theriogenology.2009.11.002
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发表时间：
2010-04-01
期刊：
影响因子：
2.8
通讯作者：
Kummer, W.
中科院分区：
文献类型：
--
作者：
Koelle, S.;Reese, S.;Kummer, W.
输卵管中配子运输、受精和早期胚胎发育的完整性是成功繁殖的必要先决条件。虽然配子运输、配子相互作用和早期胚胎发生的基本机制在大多数哺乳动物中是已知的，但配子与输卵管上皮之间的相互作用以及早期胚胎与雌性生殖道之间的通讯仍有待阐明。最近的活细胞成像技术，如数字视频显微镜和共聚焦荧光显微镜是有价值的工具，提供了实际的新的见解，这些相互作用。通过应用这些技术，可以在体内或原位条件下分析精子运输、精子储存、卵母细胞运输、配子相互作用和早期胚胎-母体串扰的机制。这些非常早期和重要的过程的详细知识创建的基础上，开发新的治疗概念，不孕不育症和改善辅助生殖技术。目前的审查将集中在一个简短的描述，最近的技术活细胞成像在生殖道随后的概述，在早期事件的生殖实际观察。(C)2010年爱思唯尔公司All rights reserved.
The integrity of gamete transport, fertilization, and early embryonic development in the oviduct are essential prerequisites for successful reproduction. Although the basic mechanisms of gamete transport, gamete interaction, and early embryogenesis are known in most mammals, the interactions between gametes and oviductal epithelium as well as the communication between the early embryo and the female reproductive tract remain to be elucidated. Recent techniques of live cell imaging such as digital videomicroscopy and confocal fluorescence microscopy are valuable tools that provide actual new insights into these interactions. By applying these techniques, the mechanisms of sperm transport, sperm storage, oocyte transport, gamete interaction, and early embryo-maternal crosstalk can be analyzed under in vivo or in situ conditions. Detailed knowledge of these very early and important processes creates the basis to develop new therapeutic concepts for subfertility and infertility and to improve the techniques of assisted reproduction. The current review will focus on a short description of recent techniques of live cell imaging in the reproductive tract followed by an overview of actual observations during the early events of reproduction. (C) 2010 Elsevier Inc. All rights reserved.