Expression of a homeobox gene product in normal and mutant zebrafish embryos: evolution of the tetrapod body plan.
Expression of a homeobox gene product in normal and mutant zebrafish embryos: evolution of the tetrapod body plan.
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发表时间：
1990-06
期刊：
影响因子：
4.6
通讯作者：
Anders Molven;Christopher V. E. Wright;R. Bremiller;E. M. D. Robertis;Charles B. Kimmel
中科院分区：
文献类型：
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作者：
Anders Molven;Christopher V. E. Wright;R. Bremiller;E. M. D. Robertis;Charles B. Kimmel
使用抗体来检测斑马鱼中似乎与含有青蛙同源结构域的蛋白XlHbox 1同源的抗原。这些抗原显示出在前后轴中的限制性表达和在胸鳍芽中的前后梯度，与XlHbox 1蛋白在青蛙和小鼠胚胎中的分布一致。在体节中胚层中，表达的前限与体节4和体节5之间的边界完全一致，蛋白质水平向后逐渐消失。在CNS的一系列Rohon-Beard感觉神经元内观察到类似的抗原分级表达。我们还对突变体spt-1（“spadetail”）进行了免疫染色，其中树干中胚层在XlHbox 1表达区域中被极大地耗尽和紊乱。这些缺陷源于原肠胚形成过程中错误的细胞运动，但神经系统，部分重新填充躯干中胚层的新招募细胞在前后轴的正常范围内表达抗原。这一发现表明，突变不会删除激活XlHbox 1基因所需的位置信息。
An antibody was used to detect antigens in zebrafish that appear to be homologous to the frog homeodomain-containing protein XlHbox 1. These antigens show a restricted expression in the anteroposterior axis and an anteroposterior gradient in the pectoral fin bud, consistent with the distribution of XlHbox 1 protein in frog and mouse embryos. In the somitic mesoderm, a sharp anterior limit of expression coincides exactly with the boundary between somites 4 and 5, and the protein level fades out posteriorly. A similar, graded expression of the antigen is seen within the series of Rohon-Beard sensory neurons of the CNS. We also immunostained the mutant spt-1 ('spadetail'), in which the trunk mesoderm is greatly depleted and disorganized in the region of XlHbox 1 expression. The defects stem from misdirected cell movements during gastrulation, but nervertheless, newly recruited cells that partially refill the trunk mesoderm express the antigen within the normal span of the anteroposterior axis. This finding suggests that the mutation does not delete positional information required for activation of the XlHbox 1 gene.