Hsa_circ_0088036 promotes the proliferation and migration of fibroblast-like synoviocytes by sponging miR-140-3p and upregulating SIRT 1 expression in rheumatoid arthritis
Hsa_circ_0088036 promotes the proliferation and migration of fibroblast-like synoviocytes by sponging miR-140-3p and upregulating SIRT 1 expression in rheumatoid arthritis
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Hsa_circ_0088036 通过海绵 miR-140-3p 和上调 SIRT 1 表达促进类风湿关节炎中成纤维样滑膜细胞的增殖和迁移
DOI：
10.1016/j.molimm.2020.07.004
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发表时间：
2020
影响因子：
3.6
通讯作者：
Yang Min
中科院分区：
文献类型：
--
作者：
Zhong Shuping;Ouyang Qingqing;Zhu Dingji;Huang Qin;Zhao Jinjun;Fan Meida;Cai Yujie;Yang Min
环状rna （circRNAs）已被证明在各种类型癌症的发生和进展中发挥关键作用，作为microRNA海绵调节靶基因的表达。尽管已经对环状rna进行了深入的研究，但它们在自身免疫性疾病（包括类风湿性关节炎（RA））中的功能和病理意义尚不清楚。我们之前的研究证实hsa_circ_0088036 （circ0088036）在RA患者外周血单个核细胞中显著升高。本研究旨在探讨circ0088036在RA发病机制中的作用。通过生物信息学工具预测circ0088036/miR-140−3p/沉默信息调节因子1 （SIRT 1）轴。与骨关节炎（OA）中的fls相比，Circ0088036在RA中的成纤维细胞样滑膜细胞（fls）中异常上调。在功能上，circ0088036的上调促进了RA-FLSs的增殖和迁移。在机制上，circ0088036作为miR-140−3p海绵上调sir1表达，随后促进RA进展。总之，本研究揭示circ0088036可能在RA中通过circ0088036/miR-140−3p/SIRT 1轴促进滑膜发病中发挥重要作用，为RA进展过程中的circRNAs提供了新的见解。
Circular RNAs (circRNAs) have been demonstrated to play crucial roles in the development and progression of various types of cancers by serving as microRNA sponges to regulate the expression of target genes. Although in-depth studies of circRNAs have been conducted, their functional and pathological significance in autoimmune diseases, including rheumatoid arthritis (RA), remains unclear. Our previous study verified that hsa_circ_0088036 (circ0088036) is significantly elevated in peripheral blood mononuclear cells from patients with RA. The present study aimed to explore the roles of circ0088036 in the pathogenesis of RA. The circ0088036/miR-140−3p/silent information regulator 1 (SIRT 1) axis was predicted by bioinformatics tools. Circ0088036 was found to be aberrantly upregulated in fibroblast-like synoviocytes (FLSs) in RA compared with FLSs in osteoarthritis (OA). Functionally, upregulated circ0088036 promoted the proliferation and migration of RA-FLSs. Mechanistically, circ0088036 acted as a miR-140−3p sponge to upregulate SIRT 1 expression, subsequently promoting RA progression. In conclusion, this study revealed that circ0088036 may play an essential role in promoting synovial pathogenesis via the circ0088036/miR-140−3p/SIRT 1 axis in RA, providing new insight into circRNAs during RA progression.