Decellularization and Recellularization Methods for Avian Lungs: An Alternative Approach for Use in Pulmonary Therapeutics.
Decellularization and Recellularization Methods for Avian Lungs: An Alternative Approach for Use in Pulmonary Therapeutics.
复制标题
禽肺的脱细胞和再细胞化方法：肺治疗中使用的替代方法。
DOI：
10.1007/978-1-0716-1811-0_33
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Uriarte,JuanJ
中科院分区：
文献类型：
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作者：
Tanneberger,AliciaE;Weiss,DanielJ;Uriarte,JuanJ
相容的同种异体器官的短缺以及在等待肺移植时需要长期肺辅助装置的患者数量的增加促使科学家探索生物工程新肺的替代方案，包括通过脱细胞和再细胞化过程。一种用于体外膜式氧合器的生物工程的新方法基于禽肺的实质结构，其利用空气和血液的交叉流单向流动而不是双向气流，并且因此消除死腔通气。这提供了比哺乳动物肺更有效的气体交换。所采用的新方法是使禽肺去细胞化，然后用患者来源的人肺上皮细胞和血管内皮细胞再细胞化，目的是产生可用作气体交换装置的全功能结构。在这里，我们提出了鸡和鸸鹋肺的禽肺脱细胞化和再细胞化方法，以研究小规模和大规模的禽肺模型。对于去细胞化，利用基于去污剂的方案，并使用不同的技术来验证这些肺的去细胞化和再细胞化，包括显微镜、质谱和免疫组织化学分析。对于再细胞化，提出了将不同的人肺细胞类型接种到脱细胞支架中的技术。
The shortage of compatible allogeneic organs and an increase in the number of patients requiring long-term lung assist devices while waiting for lung transplantation have motivated scientists to explore alternatives to bioengineer new lungs, including through decellularization and recellularization processes. A novel approach for bioengineering an extracorporeal membrane oxygenator is based on the parenchymal structure of avian lungs which utilizes a cross-current unidirectional flow of air and blood rather than bidirectional airflow, and thus eliminates dead-space ventilation. This provides more efficient gas exchange than mammalian lungs. The novel approach utilized is to decellularize avian lungs and then to recellularize with patient-derived human lung epithelial and vascular endothelial cells with the goal of creating a fully functional structure that can be used as a gas-exchange device. Here, we present avian lung decellularization and recellularization methods for chicken and emu lungs, in order to study both small- and large-scale avian lung models. For decellularization, a detergent-based protocol is utilized, and different techniques are used to validate the de- and recellularization of those lungs, including microscopy, mass spectrometry, and immunohistochemical analyses. For recellularization, techniques for seeding different human lung cell types into the decellularized scaffolds are presented.