Specificity and efficiency of cre-mediated recombination in Emx1-Cre knock-in mice
Specificity and efficiency of cre-mediated recombination in Emx1-Cre knock-in mice
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DOI：
10.1006/bbrc.2000.2870
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发表时间：
2000-07-05
影响因子：
3.1
通讯作者：
Li, YQ
中科院分区：
文献类型：
--
作者：
Guo, HL;Hong, SS;Li, YQ
Emx1是果蝇同源盒基因空气门的小鼠同源物，其表达仅限于发育和成年大脑皮层和海马中的神经元。我们之前报道了使用胚胎干细胞技术将cre基因直接置于Emx1启动子下游的转基因小鼠的创建。我们发现Cre蛋白存在于转基因小鼠的大脑皮层中，并且能够在体外介导loxp特异性重组。在本研究中，我们使用三种独立的报告小鼠系，结合组织化学染色、神经元培养和基因组DNA的Southern检测来确定cre介导重组的特异性和效率。我们的结果表明，重组在所有三种被测试的报告小鼠系中都是高效的，并证实了缺失仅限于大脑皮层和海马中的神经元。此外，我们已经确定大脑皮层的重组效率为91%。我们的研究结果表明，Emx1并不是在发育和成年大脑皮层的每个神经元中都表达。这一cre小鼠系将有助于皮质发育和可塑性的研究。(C) 2000年学术出版社。
Emx1 is a mouse homologue of the Drosophila homeobox gene empty spiracles and its expression is restricted to the neurons in the developing and adult cerebral cortex and hippocampus. We reported previously the creation of a line of transgenic mice in which the cre gene was placed directly downstream of the putative Emx1 promoter using ES cell technology. We showed that Cre protein was present in the cerebral cortex of the transgenic mice and was able to mediate loxP-specific recombination in vitro. In the present study, the specificity and efficiency of the cremediated recombination were determined using three independent lines of reporter mice and a combination of histochemical staining, neuronal culture, and Southern detection of the genomic DNA, Our results showed that the recombination was highly efficient in all three lines of reporter mice tested and confirmed that the deletion was restricted to the neurons in the cerebral cortex and hippocampus. Furthermore, we have determined that the recombination efficiency in the cerebral cortex was 91%. Our results suggest that Emx1 is not expressed in every neuron in the developing and adult cerebral cortex. This line of cre mice should contribute to the studies of cortical development and plasticity. (C) 2000 Academic Press.