A newly developed bioartificial pancreas successfully controls blood glucose in totally pancreatectomized diabetic pigs.
A newly developed bioartificial pancreas successfully controls blood glucose in totally pancreatectomized diabetic pigs.
复制标题
新开发的生物人工胰腺成功地控制了完全切除胰腺的糖尿病猪的血糖。
DOI：
10.1089/ten.2006.12.1799
复制
发表时间：
2006
影响因子：
--
通讯作者：
J. Yoon
中科院分区：
文献类型：
--
作者：
H. Ikeda;N. Kobayashi;Yoshihito Tanaka;S. Nakaji;Chen Yong;T. Okitsu;M. Oshita;S. Matsumoto;H. Noguchi;M. Narushima;Kimiaki Tanaka;A. Miki;J. Rivas;A. Soto‐Gutierrez;N. Navarro;Koichi Tanaka;H. Jun;N. Tanaka;J. Yoon
构建安全、功能良好的生物人工胰腺（BAP），为胰岛细胞提供良好的生长环境，可能是治疗糖尿病的重要途径。已经开发了各种类型的BAP装置，但是它们中的大多数涉及微囊或扩散室中的胰岛的血管外植入。这些装置在胰岛和血液之间具有差的扩散交换，并且经常破裂。为了克服这些问题，我们开发了一种新型的BAP，由可渗透葡萄糖和胰岛素的聚乙烯-乙烯醇（EVAL）中空纤维和允许细胞粘附的聚氨基甲酸酯涂层非织造聚四氟乙烯（PTFE）织物组成。猪胰岛附着在PTFE织物的表面，但不附着在EVAL中空纤维的表面，允许纤维内部流动的血液与外部细胞之间进行营养和氧气交换。我们用猪胰岛接种这种BAP，并将其连接到完全切除胰腺的糖尿病猪的循环中。我们发现血糖水平降低到正常范围，总体健康状况得到改善，从而延长了生存时间。此外，在体外和体内，BAP的胰岛素分泌调节响应于葡萄糖而被适当地控制。这些结果表明，我们新开发的BAP可能是治疗人类糖尿病的潜在疗法。
Construction of a safe and functional bioartificial pancreas (BAP) that provides an adequate environment for islet cells may be an important approach to treating diabetic patients. Various types of BAP devices have been developed, but most of them involve extravascular implantation of islets in microcapsules or diffusion chambers. These devices have poor diffusive exchange between the islets and blood, and often rupture. To overcome these problems, we developed a new type of BAP composed of polyethylene-vinyl alcohol (EVAL) hollow fibers that are permeable to glucose and insulin and a poly-amino-urethane-coated, non-woven polytetrafluoroethylene (PTFE) fabric that allows cell adhesion. Porcine islets attached to the surface of the PTFE fabric, but not to the surface of the EVAL hollow fibers, allowing nutrient and oxygen exchange between blood flowing inside the fibers and cells outside. We inoculated this BAP with porcine islets and connected it to the circulation of totally pancreatectomized diabetic pigs. We found that blood glucose levels were reduced to a normal range and general health was improved, resulting in longer survival times. In addition, regulation of insulin secretion from the BAP was properly controlled in response to glucose both in vitro and in vivo. These results indicate that our newly developed BAP may be a potential therapy for the treatment of diabetes in humans.