Characterization of the metalloproteome of Pseudoalteromonas (BB2-AT2): biogeochemical underpinnings for zinc, manganese, cobalt, and nickel cycling in a ubiquitous marine heterotroph.
Characterization of the metalloproteome of Pseudoalteromonas (BB2-AT2): biogeochemical underpinnings for zinc, manganese, cobalt, and nickel cycling in a ubiquitous marine heterotroph.
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假交替单胞菌 (BB2-AT2) 金属蛋白质组的表征：普遍存在的海洋异养生物中锌、锰、钴和镍循环的生物地球化学基础。
DOI：
10.1093/mtomcs/mfab060
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Saito,MakA
中科院分区：
文献类型：
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作者：
Mazzotta,MichaelG;McIlvin,MatthewR;Moran,DawnM;Wang,DavidT;Bidle,KayD;Lamborg,CarlH;Saito,MakA
假交替单胞菌（Pseudoalteromonas，BB 2-AT 2）是一种普遍存在的海洋异养生物，通常与海洋中不稳定的有机碳源（如浮游植物水华和沉降颗粒）有关。异养生物使用含有锌（Zn）、锰（Mn）、钴（Co）和镍（Ni）的多种金属酶水解输出的光合材料（生物碳泵的组分）。尽管海洋异养生物对金属的需求和细胞溶质对金属的利用与全球碳循环有关，但对它们的研究很少。在这里，我们表征了BB 2-AT 2的Zn、Mn、Co和Ni金属组。我们发现，锌金属组是复杂的和胞质锌与许多蛋白质的转录（47.2%的金属组，从金属蛋白组学数据的奇异值分解），翻译（33.5%），蛋白水解（12.8%），和碱性磷酸酶活性（6.4%）。许多蛋白水解酶也似乎是pupelium与锰，并在较小程度上，公司推定鉴定镍相关的蛋白质，磷酸葡萄糖变位酶和cupin超家族中的蛋白质，提供了新的见解镍利用海洋异养生物。BB 2-AT 2依赖于许多过渡金属的蛋白水解和磷酸酶活性，推断出对金属限制的适应潜力。我们的实地观察，增加碱性磷酸酶活性后，在现场孵化锌表明，这种金属的限制，在下沉颗粒物质收集的沉积物陷阱。总之，这项研究提高了我们对海洋异养细菌的锌，锰，钴，镍金属组的理解，并为理解营养限制对海洋地球化学循环的影响提供了新的机制框架。
Pseudoalteromonas(BB2-AT2) is a ubiquitous marine heterotroph, often associated with labile organic carbon sources in the ocean (e.g. phytoplankton blooms and sinking particles). Heterotrophs hydrolyze exported photosynthetic materials, components of the biological carbon pump, with the use of diverse metalloenzymes containing zinc (Zn), manganese (Mn), cobalt (Co), and nickel (Ni). Studies on the metal requirements and cytosolic utilization of metals for marine heterotrophs are scarce, despite their relevance to global carbon cycling. Here, we characterized the Zn, Mn, Co, and Ni metallome of BB2-AT2. We found that the Zn metallome is complex and cytosolic Zn is associated with numerous proteins for transcription (47.2% of the metallome, obtained from singular value decomposition of the metalloproteomic data), translation (33.5%), proteolysis (12.8%), and alkaline phosphatase activity (6.4%). Numerous proteolytic enzymes also appear to be putatively associated with Mn, and to a lesser extent, Co. Putative identification of the Ni-associated proteins, phosphoglucomutase and a protein in the cupin superfamily, provides new insights for Ni utilization in marine heterotrophs. BB2-AT2 relies on numerous transition metals for proteolytic and phosphatase activities, inferring an adaptative potential to metal limitation. Our field observations of increased alkaline phosphatase activity upon addition of Zn in field incubations suggest that such metal limitation operates in sinking particulate material collected from sediment traps. Taken together, this study improves our understanding of the Zn, Mn, Co, and Ni metallome of marine heterotrophic bacteria and provides novel and mechanistic frameworks for understanding the influence of nutrient limitation on biogeochemical cycling.