Influence of administration methods on the accumulation of ALA-induced Pp-IX in mouse tongue tumors
Influence of administration methods on the accumulation of ALA-induced Pp-IX in mouse tongue tumors
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DOI：
10.1111/j.1601-0825.2006.01216.x
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发表时间：
2006-07-01
期刊：
影响因子：
3.8
通讯作者：
Kinoshita, H.
中科院分区：
文献类型：
--
作者：
Ogasawara, T.;Miyoshi, N.;Kinoshita, H.
目的：5-氨基乙酰丙酸（ALA）诱导的原卟啉IX（PpIX）已被用作光动力疗法（PDT）中的光敏剂用于口腔癌。本研究通过分析舌肿瘤组织中PpIX的荧光来探讨ALA的最佳给药方法。方法：将小鼠（C3H）移植的舌癌（NR-S1）中的原卟啉IX强度与腹腔注射（i.p.）后正常舌中的原卟啉IX强度进行比较，经口（p.o.）或局部施用ALA。在ALA给药后的不同时间对舌头取样。结果：肿瘤组原卟啉IX强度在腹腔注射后3h和腹腔注射后5h达到峰值，在腹腔注射后3h和腹腔注射后5h达到峰值。给予ALA后，这些水平约为正常组的两倍。舌肿瘤组织中的最大PpIX积累在p.o. ALA的管理。相反，局部施用20%ALA乳膏与整个实验中肿瘤中最低的PpIX积累相关。ALA是ALA-PDT治疗口腔癌最有效的方法。
OBJECTIVE: 5-Aminolevulinic acid (ALA)-induced protoporphyrin IX (PpIX) has been used as a photosensitizer in photodynamic therapy (PDT) for oral cancer. This study investigates the optimal method of administrating ALA by analyzing PpIX fluorescence in tongue tumor tissue.METHODS: Protoporphyrin IX intensities in the mouse (C3H)-transplanted tongue cancer (NR-S1) were compared with those in normal tongue after intraperitoneal (i.p.), oral (p.o.) or topical administration of ALA. Tongues were sampled at various times after ALA administration. PpIX intensities were obtained from frozen sections of each sample by using a spectrophotometer.RESULTS: Protoporphyrin IX intensity in the tumor group peaked at 3 h after the i.p. and 5 h after the p.o. administration of ALA, and these levels were about twice as high as those in the normal group. Maximum PpIX accumulation in the tongue tumor tissue was seen at 5 h after p.o. administration of ALA. In contrast, the topical administration of 20% ALA cream was associated with the lowest PpIX accumulation in the tumor throughout the experiments.CONCLUSION: These results suggested that p.o. administration of ALA was the most effective method in ALA-PDT for oral cancer.