Designable Immune Therapeutical Vaccine System Based on DNA Supramolecular Hydrogels
Designable Immune Therapeutical Vaccine System Based on DNA Supramolecular Hydrogels
复制标题
基于DNA超分子水凝胶的可设计免疫治疗疫苗系统
DOI：
10.1021/acsami.8b00312
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发表时间：
2018
影响因子：
9.5
通讯作者：
Li Yan Mei
中科院分区：
文献类型：
--
作者：
Shao Yu;Sun Zhan Yi;Wang Yijie;Zhang Bo Dou;Liu Dongsheng;Li Yan Mei
免疫治疗可以打破肿瘤的免疫耐受，诱导机体产生强有力的免疫应答，被认为是治疗肿瘤的理想方法。然而，构建一个广泛的抗原适应性，易于处理，可生物降解的系统，可以招募和激活抗原呈递细胞（APC）非常有效的仍然是一个挑战。在此，我们展示了一种可注射的DNA超分子水凝胶疫苗（DSHV）系统，该系统可以在体外和体内有效地招募和激活APC。在体外过程中已经可视化的荧光显微镜。通过腹膜内或皮下注射，DSHV系统可以模拟淋巴结的功能，其中APC被高局部浓度的胞嘧啶-磷酸-鸟嘌呤募集和激活。随后，获得了较强的免疫应答和明显的抗肿瘤作用。我们的研究结果表明，DSHV系统可以作为肿瘤疫苗接种的通用平台，并在不久的将来有利于个性化的癌症治疗。
Immunotherapy is believed to be an ideal method to treat cancer because it can break the immunotolerance of tumor and induce robust immunoresponse. However, constructing a wide antigen-adaptive, easy-handling, and biodegradable system that can recruit and activate antigen-presenting cells (APCs) much effectively is still a challenge. Herein, we show an injectable DNA supramolecular hydrogel vaccine (DSHV) system which could efficiently recruit and activate APCs in vitro and in vivo. The in vitro processes have been visualized by fluorescence microscopy. Through intraperitoneal or subcutaneous injection, the DSHV system can mimic the function of a lymph node where the APCs are recruited and activated by the high local concentration of cytosine-phosphate-guanine. Subsequently, strong immune response and obvious antitumor effects have been obtained. Our findings demonstrated that the DSHV system could serve as a general platform for tumor vaccination and benefit the personalized cancer therapy in the near future.