Inhibition of proliferation of human Hela cells by small interference RNA against Pokemon gene
Inhibition of proliferation of human Hela cells by small interference RNA against Pokemon gene
复制标题
Pokemon基因小干扰RNA抑制人Hela细胞增殖
DOI：
10.1007/s11670-008-0005-4
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发表时间：
2008-03-01
影响因子：
5.1
通讯作者：
Wu Yu-zhang
中科院分区：
文献类型：
--
作者：
Deng Yi-jing;Ni Bing;Wu Yu-zhang
Objective: The transcriptional repressor Pokemon (encoded by the Zbtb7 gene) is a critical factor in oncogenesis. Pokemon overexpression leads to overt oncogenic transformation both in vitro and in vivo in transgenic mice. The objective of this study was to investigate the effect of retrovirus expressing the siRNA targeting Pokemon in human cervical cancer cells. Methods: We constructed and identified the recombinant retrovirus particle expressing siRNA of Pokemon gene, and then testified the suppression of recombinant plasmid and evaluated the gene-silencing effect. Results: We got the positive evaluation from colony forming experiment we found that the retrovirus expressing siRNA targeting Pokemon had repressing effect. Conclusion: Our work provides basis for the study of suppression effect of retrovirus in vivo and the design of the target-complex.
