Encapsulation of Chlamydomonas reinhardtii into a metal-phenolic network
Encapsulation of Chlamydomonas reinhardtii into a metal-phenolic network
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DOI：
10.1016/j.algal.2021.102569
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发表时间：
2021-11
期刊：
影响因子：
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通讯作者：
N. Mandsberg;Wenting Liao;Yoshihiro Alexander Yamanouchi;A. Boisen;H. Ejima
中科院分区：
文献类型：
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作者：
N. Mandsberg;Wenting Liao;Yoshihiro Alexander Yamanouchi;A. Boisen;H. Ejima
使用金属酚网络（MPN）的单细胞封装是一种保护细胞免受压力源影响的新兴技术。到目前为止，MPN 涂层仅应用于某些细菌、酵母和哺乳动物细胞。为了进一步证明它们的多功能性，我们在此用由单宁酸和铁（III）组成的网络涂覆莱茵衣藻。封装作为机械屏障，延迟了包被细胞的增殖。此外，我们研究了单宁酸在C表面的吸附。莱因哈蒂安确定了实现藻类表面部分覆盖的最佳浓度。最后，我们探讨了 MPN 涂层对絮凝增强藻类收获的影响。总体而言，结果表明使用金属酚网络的电池封装技术适用于 C。莱茵哈特（reinhardtii），在能量收集和生物医学应用的绿藻表面工程方面很有前景。
Single-cell encapsulation using metal-phenolic networks (MPNs) is an emerging technique to protect cells from stressors. Until now, the MPN coatings have been only applied to some bacteria, yeast, and mammalian cells. To further demonstrate their versatility, we herein coatChlamydomonas reinhardtiiwith the network consisting of tannic acid and iron(III). The encapsulation served as a mechanical barrier, which delayed proliferation of the coated cells. Furthermore, we investigated the adsorption of tannic acid onto the surface ofC. reinhardtiiand identified the optimum concentration to achieve partial coverage on the algae's surface. Finally, we explored the effect of MPN coating for flocculation-enhanced algae harvesting. Overall, the results demonstrate that the cell encapsulation technique using metal-phenolic networks is applicable to theC. reinhardtii, which is promising for the surface engineering of the green algae for energy harvesting and biomedical applications.