Excretion/secretion products from Schistosoma mansoni adults, eggs and schistosomula have unique peptidase specificity profiles.
Excretion/secretion products from Schistosoma mansoni adults, eggs and schistosomula have unique peptidase specificity profiles.
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DOI：
10.1016/j.biochi.2015.09.025
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发表时间：
2016-03
期刊：
影响因子：
3.9
通讯作者：
O'Donoghue AJ
中科院分区：
文献类型：
--
作者：
Dvořák J;Fajtová P;Ulrychová L;Leontovyč A;Rojo-Arreola L;Suzuki BM;Horn M;Mareš M;Craik CS;Caffrey CR;O'Donoghue AJ
血吸虫病是严重影响个人和社会福祉及生产力的贫穷慢性蠕虫病之一。肽酶（蛋白酶）是成功寄生的关键，可以调节宿主的生理和免疫。特定药物或疫苗对肽酶作用的干扰可以是治疗上有益的。到目前为止，对寄生虫中肽酶的研究主要集中在单个肽酶的功能表征或作为全球基因组或转录组研究的一部分的注释上。我们有兴趣在功能上表征肽酶活性的复杂性，在宿主-寄生虫界面，因此，排泄分泌产物的关键发育阶段的曼氏血吸虫寄生在人类进行了检查。使用类特异性肽酶抑制剂与多重底物分析相结合，一些独特的活动，来自内切和外切肽酶被发现在排泄分泌产物的童虫（幼虫迁飞蠕虫），成人和鸡蛋。这些数据强调了这种寄生虫每个发育阶段功能降解组的复杂性，并有助于进一步研究以确定肽酶身份、生理和免疫功能以及作为候选药物或疫苗的实用性。
Schistosomiasis is one of a number of chronic helminth diseases of poverty that severely impact personal and societal well-being and productivity. Peptidases (proteases) are vital to successful parasitism, and can modulate host physiology and immunology. Interference of peptidase action by specific drugs or vaccines can be therapeutically beneficial. To date, research on peptidases in the schistosome parasite has focused on either the functional characterization of individual peptidases or their annotation as part of global genome or transcriptome studies. We were interested in functionally characterizing the complexity of peptidase activity operating at the host-parasite interface, therefore the excretory-secretory products of key developmental stages of Schistosoma mansoni that parasitize the human were examined. Using class specific peptidase inhibitors in combination with a multiplex substrate profiling assay, a number of unique activities derived from endo- and exo-peptidases were revealed in the excretory-secretory products of schistosomula (larval migratory worms), adults and eggs. The data highlight the complexity of the functional degradome for each developmental stage of this parasite and facilitate further enquiry to establish peptidase identity, physiological and immunological function, and utility as drug or vaccine candidates.