BASAL PROMOTER ELEMENTS AS A SELECTIVE DETERMINANT OF TRANSCRIPTIONAL ACTIVATOR FUNCTION
BASAL PROMOTER ELEMENTS AS A SELECTIVE DETERMINANT OF TRANSCRIPTIONAL ACTIVATOR FUNCTION
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DOI：
10.1038/374657a0
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发表时间：
1995-04-13
期刊：
影响因子：
64.8
通讯作者：
HERR, W
中科院分区：
文献类型：
--
作者：
DAS, G;HINKLEY, CS;HERR, W
在真核生物中，转录的激活涉及与顺式调控元件结合的激活物和与转录起始部位附近的基本元件结合的因子之间的相互作用。基本元件，例如小核糖核酸(SnRNA)启动子的TATA盒或近端序列元件(PSE)，使基本转录复合体的组装成为核，其组件与激活剂(1，2)相互作用。虽然一个碱基转录复合体可以与许多激活剂相互作用，但不同的碱基转录复合体是否能对特定的激活剂产生不同的反应尚不清楚。我们在这里表明，改变基本元素的排列可以改变对转录激活域的反应。事实上，在人类U6SnRNA启动子中，TATA盒或PSE的点突变导致对VP16和Sp1衍生的激活域的完全相反的反应。这些基本元素甚至可以区分激活域中的微小变化。因此，基本启动子元件的排列为转录的差异调控提供了一种机制。
IN eukaryotes, activation of transcription involves an interplay between activators bound to cis-regulatory elements and factors bound to basal elements near the start site of transcription. The basal elements, for example the TATA box or proximal sequence element (PSE) of small nuclear RNA (snRNA) promoters, nucleate the assembly of basal transcription complexes, components of which interact with activators(1,2). Although one basal transcription complex can interact with many activators, it is unclear whether different basal transcription complexes can direct different responses to particular activators. We show here that changing the arrangement of basal elements can alter the response to transcriptional activation domains. Indeed, in the human U6 snRNA promoter, point mutation of either a TATA box or PSE results in diametrically opposed responses to VP16- and Sp1-derived activation domains. These basal elements can even discriminate small changes in an activation domain. Thus the arrangement of basal promoter elements provides a mechanism for differential regulation of transcription.