Proliferation of Saos-2 and Ca9-22 cells on grooved and pillared titanium surfaces
Proliferation of Saos-2 and Ca9-22 cells on grooved and pillared titanium surfaces
复制标题
DOI：
10.3233/bme-191074
复制
发表时间：
2020-01-01
影响因子：
1
通讯作者：
Yoshida, Yasuhiro
中科院分区：
文献类型：
--
作者：
Kaga, Naoyuki;Akasaka, Tsukasa;Yoshida, Yasuhiro
背景：钛（Ti）口腔种植体的表面纳米结构对快速骨整合至关重要。目的：研究成骨样细胞（Saos-2）和上皮样细胞（Ca9-22）在纳米钛膜上的生长情况。方法：采用纳米印迹技术制备具有500 nm凹槽和柱状结构的Ti薄膜，并分别播种Saos-2和Ca9-22细胞。分别用细胞增殖试验和扫描电镜观察细胞活力和形态。结果：1小时后，Saos-2细胞在沟槽膜上的增殖比柱状膜和光滑膜上的增殖更强劲。24小时和5天后，这些细胞在沟槽和柱状基质上大约翻倍，但在光滑表面上没有。相比之下，Ca9-22细胞偏爱光滑的表面，其次是凹槽和柱状薄膜。确实，在5天的培养过程中，细胞稀疏地粘附在柱状膜上（p < 0.05）。结论：这些数据表明Saos-2和Ca9-22细胞对不同纳米结构的反应不同，并强调了纳米模式在促进骨再生或防止种植体-骨界面上皮细胞生长下降方面的潜在应用。
BACKGROUND: Surface nanostructures in titanium (Ti) oral implants are critical for rapid osseointegration.OBJECTIVE: The purpose of this study was to evaluate the growth of osteoblast-like (Saos-2) and epithelial-like (Ca9-22) cells on nanopatterned Ti films.METHODS: Ti films with 500 nm grooves and pillars were fabricated by nanoimprinting, and seeded with Saos-2 and Ca9-22 cells. Cell viability and morphology were assessed by cell proliferation assay and scanning electron microscopy, respectively.RESULTS: As assessed after 1 hour, proliferation of Saos-2 cells was most robust on grooved films than on pillared and smooth films, in this order. These cells approximately doubled on grooved and pillared substrates in 24 hours and after 5 days, but not on smooth surfaces. In contrast, Ca9-22 cells favored smooth surfaces, followed by grooved and pillared films. Indeed, cells sparsely adhered to pillared films over 5 days of incubation (p < 0.05).CONCLUSIONS: The data show that Saos-2 and Ca9-22 cells respond differently to different nanostructures, and highlight the potential use of nanopatterns to promote bone regeneration or to prevent epithelial downgrowth at the implant-bone interface.