Monitoring the enzyme expression in a respiratory chain of Corynebacterium glutamicum in a copper ion-supplemented culture medium.
Monitoring the enzyme expression in a respiratory chain of Corynebacterium glutamicum in a copper ion-supplemented culture medium.
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在补充铜离子的培养基中监测谷氨酸棒杆菌呼吸链中的酶表达。
DOI：
10.1080/09168451.2014.968089
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Sakamoto J
中科院分区：
文献类型：
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作者：
Kusumoto T;Aoyagi M ;Sugiyama T;Sakamoto J
谷氨酸棒杆菌具有分支呼吸链：其中一个分支是细胞色素bcc复合物和细胞色素a 3型细胞色素氧化酶，另一个分支是细胞色素b d型甲萘醌氧化酶。影响这些呼吸酶表达模式的因素仍不清楚。为了研究这些酶的表达调控机制，我们以前构建了一个利用增强型绿色荧光蛋白的启动子检测系统。在这里，我们监测呼吸酶的表达，通过使用该系统在各种培养基中的生长过程中，有和没有Cu 2+离子补充。在稳定期早期，添加40或400 μM的Cu ~（2+），细胞色素a_3氧化酶的启动子活性分别比对照提高了1.49倍和1.99倍。此外，H+/O比，或质子泵活性的细胞，增加了约1.6倍的Cu 2+补充。这些事实表明，铜离子可以切换分支。
Corynebacterium glutamicumhas a branched respiratory chain: one of the branches is cytochromebcccomplex and cytochromeaa3-type cytochromecoxidase, and the other is cytochromebd-type menaquinol oxidase. The factors that influence the expression patterns of these respiratory enzymes remain unclear. To investigate the expressional control mechanism of the enzymes, we have previously constructed a promoter assay system utilizing enhanced green fluorescence protein. Here, we monitored respiratory enzymes’ expression by using this system during growth in various culture media, with and without Cu2+ion supplementation. The promoter activities of cytochromeaa3oxidase in the early stationary phase in the media supplemented with Cu2+ion at 40 or 400 μM were significantly increased 1.49-fold or 1.99-fold, respectively, as compared to the control. Moreover, the H+/O ratio, or the proton-pumping activity of the cells, increased about 1.6 times by the Cu2+supplementation. These facts indicate that copper ions can switch the branches.