Enhanced expression of plasmid DNA-cationized gelatin complex by ultrasound in murine muscle (Retracted article. See vol. 232, pg. 267, 2016)
Enhanced expression of plasmid DNA-cationized gelatin complex by ultrasound in murine muscle (Retracted article. See vol. 232, pg. 267, 2016)
复制标题
DOI：
10.1016/s0168-3659(02)00029-9
复制
发表时间：
2002-04-23
影响因子：
10.8
通讯作者：
Tabata, Y
中科院分区：
文献类型：
--
作者：
Aoyama, T;Hosseinkhani, H;Tabata, Y
本研究是一项调查，以实验证实，超声（US）照射是有效的，在提高质粒DNA的基因表达。通过在明胶中引入伯氨基，制备了一种阳离子明胶。将LacZ基因的质粒与阳离子化明胶复合并注射到正常小鼠的股肌中。在肌肉的注射部位进行US照射后，评价处理的肌肉的基因表达，以与复合物注射加无US照射的基因表达进行比较。与裸质粒DNA相比，US照射使复合物能够显着增强注射肌肉的基因表达。尽管其程度取决于实验条件，如质粒DNA的注射剂量和US照射的时间段或时机。荧光显微镜观察显示，复合物均匀分布于注射部位周围的肌肉组织中，质粒DNA没有发生解离。质粒DNA的荧光图像与肌球蛋白重链的荧光图像重叠，表明质粒DNA的细胞内定位。我们证明，US照射是一种很有前途的技术，以提高基因表达，即使在正常的肌肉。(C)2002 Elsevier Science B.V.保留所有权利。
This study is an investigation to experimentally confirm that ultrasound (US) irradiation is effective in enhancing the gene expression of plasmid DNA. A cationized gelatin was prepared by introducing primary amino groups into gelatin. The plasmid of the LacZ gene was complexed with the cationized gelatin and injected into the femoral muscle of normal mice. Following US irradiation at the injected site of muscle, the gene expression of the treated muscle was evaluated to compare with that of the complex injection plus no US irradiation. US irradiation enabled the complex to significantly enhance the gene expression at the injected muscle, in contrast to naked plasmid DNA. although the extent depended on the experimental conditions, such as the injection dose of plasmid DNA, and the time period or timing of US irradiation. The gene-expressed area at the muscle was not changed by the time interval between the complex injection and US irradiation, Fluorescent microscopic observation revealed that the complex was homogeneously distributed in the muscle tissue around the injected site by US irradiation without any dissociation of plasmid DNA. The fluorescent image of plasmid DNA superposed on that of myosin heavy chain indicated intracellular localization of plasmid DNA. We demonstrated that US irradiation is a promising technique to enhance gene expression even in the normal muscle. (C) 2002 Elsevier Science B.V. All rights reserved.