Debaryomyces hansenii (Candida famata), a rare human fungal pathogen often misidentified as Pichia guilliermondii (Candida guilliermondii)
Debaryomyces hansenii (Candida famata), a rare human fungal pathogen often misidentified as Pichia guilliermondii (Candida guilliermondii)
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DOI：
10.1128/jcm.01451-08
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发表时间：
2008-10-01
影响因子：
9.4
通讯作者：
Dromer, Francoise
中科院分区：
文献类型：
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作者：
Desnos-Ollivier, Marie;Ragon, Marie;Dromer, Francoise
汉逊德巴利酵母（Debaryomyces hansenii）是一种半子囊酵母，常见于天然基质和各种类型的奶酪中。季也蒙毕赤酵母在自然界中广泛分布，是正常人体微生物群落的常见组成部分。这两个物种在人类感染中均有描述，但在表型上极难区分。因此，识别中经常出现错误。2000年至2007年间，62株临床和环境分离株被送往法国国家真菌病和抗真菌药参考中心，作为D。通过碳同化模式、假菌丝的存在以及rRNA基因的ITS和D1/D2区的测序分析了汉逊青霉和季也蒙青霉。本研究的目的是使用核苷酸序列评估表型鉴定是否准确，以及在测序不可用时，表型特征是否可用于区分两个物种。结果发现，58%的分离物被错误鉴定，分属于7个不同的种：季也蒙毕赤酵母、加勒比毕赤酵母、贾氏毕赤酵母、杜氏毕赤酵母。hansenii、Candida palmioleophila、C. haemulonii II型和Clavispora lusitaniae。最后，D.汉逊氏菌可能不像先前认为的那样是人类常见的病原体。ITS区或D1/D2区的测序是区分常与D. hansenii，牢记应使用可靠的数据库。
Debaryomyces hansenii is a hemiascomycetous yeast commonly found in natural substrates and in various types of cheese. Pichia guilliermondii is widely distributed in nature and is a common constituent of the normal human microflora. Both species have been described in human infections but are extremely difficult to differentiate phenotypically. Thus, frequent errors in identification occur. The 62 clinical and environmental isolates sent between 2000 and 2007 to the French National Reference Center for Mycoses and Antifungals as D. hansenii or P. guilliermondii were analyzed by using the carbon assimilation pattern, the presence of pseudohyphae, and sequencing of the ITS and D1/D2 regions of the rRNA gene. The objective of this study was to assess using nucleotide sequences whether phenotypic identification was accurate and whether phenotypic characteristics could be used to differentiate the two species when sequencing was not available. We found that 58% of the isolates were misidentified and belong to seven different species: P. guilliermondii, P. caribbica, P. jadinii, D. hansenii, Candida palmioleophila, C. haemulonii type II, and Clavispora lusitaniae. In conclusion, D. hansenii may not be as common a human pathogen as previously thought. Sequencing of either ITS or D1/D2 regions is a good tool for differentiating the species more frequently confused with D. hansenii, keeping in mind that reliable databases should be used.