Hydrogen sulfide is involved in the chilling stress response in Vitis vinifera L.
Hydrogen sulfide is involved in the chilling stress response in Vitis vinifera L.
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DOI：
10.5586/asbp.2013.031
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发表时间：
2013-01-01
影响因子：
1
通讯作者：
Liu, Xin
中科院分区：
文献类型：
--
作者：
Fu, Peining;Wang, Wenjie;Liu, Xin
硫化氢（H2S）是一种重要的信号分子，参与植物的多种抗逆过程，例如干旱和重金属胁迫。然而，人们对 H2S 在应对冷胁迫中的作用知之甚少。在本文中，我们证明低温胁迫增强了葡萄'F-242'中的H2S水平、H2S合成酶（L-/D-半胱氨酸脱硫酶，L/DCD）活性以及L/DCD基因的表达。此外，在4℃下用硫氢化钠（NaHS，H2S供体）和亚牛磺酸（HT，H2S清除剂）处理幼苗，以检查外源H2S对葡萄的影响。结果表明，添加NaHS后，超氧化物歧化酶活性升高，VvICE1和VvCBF3基因表达增强，但超氧阴离子自由基水平、丙二醛含量和细胞膜通透性降低。相反，HT处理在冷却温度下表现出相反的效果。综上所述，这些数据表明H2S可能直接参与葡萄的冷信号转导途径。
Hydrogen sulfide (H2S) is an important signaling molecule involved in several stress-resistance processes in plants, such as drought and heavy metal stresses. However, little is known about the roles of H2S in responses to chilling stress. In this paper, we demonstrated that chilling stress enhance the H2S levels, the H2S synthetase (L-/D-cysteine desulfhydrase, L/DCD) activities, and the expression of L/DCD gene in Vitis vinifera L. 'F-242'. Furthermore, the seedlings were treated with sodium hydrosulfide (NaHS, a H2S donor) and hypotaurine (HT, a H2S scavenger) at 4 degrees C to examine the effects of exogenous H2S on grape. The results revealed that the high activity of superoxide dismutase and enhanced expression of VvICE1 and VvCBF3 genes, but low level of superoxide anion radical, malondialdehyde content and cell membrane permeability were detected after addition of NaHS. In contrast, HT treatment displayed contrary effect under the chilling temperature. Taken together, these data suggested that H2S might be directly involved in the cold signal transduction pathway of grape.