Giant Acceleration of Diffusion Observed in a Single-Molecule Experiment on F(1)-ATPase.
Giant Acceleration of Diffusion Observed in a Single-Molecule Experiment on F(1)-ATPase.
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DOI：
10.1103/physrevlett.114.248101
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发表时间：
2015-06
影响因子：
8.6
通讯作者：
Ryunosuke Hayashi;K. Sasaki;Shuichi Nakamura;S. Kudo;Y. Inoue;H. Noji;K. Hayashi
中科院分区：
文献类型：
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作者：
Ryunosuke Hayashi;K. Sasaki;Shuichi Nakamura;S. Kudo;Y. Inoue;H. Noji;K. Hayashi
扩散的巨加速（GA）是由Reimann等人[Phys.Rev.Lett. 87，010602（2001）]。在这里，我们将扩散的GA理论应用于旋转马达蛋白F（1）的单分子实验，F（1）是F（o）F（1）腺苷三磷酸合酶的一个组成部分。我们讨论了F（1）的能量性质，并确定了在腺苷二磷酸与F（1）催化中心紧密结合的条件下，旋转势的高能垒为20 k（B）T.总之，扩散的遗传算法是有用的测量非平衡和单分子实验中的能垒。
The giant acceleration (GA) of diffusion is a universal phenomenon predicted by the theoretical analysis given by Reimann et al. [Phys. Rev. Lett. 87, 010602 (2001)]. Here we apply the theory of the GA of diffusion to a single-molecule experiment on a rotary motor protein, F(1), which is a component of F(o)F(1) adenosine triphosphate synthase. We discuss the energetic properties of F(1) and identify a high energy barrier of the rotary potential to be 20k(B)T, with the condition that the adenosine diphosphates are tightly bound to the F(1) catalytic sites. To conclude, the GA of diffusion is useful for measuring energy barriers in nonequilibrium and single-molecule experiments.