SUBTYPES OF INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR IN HUMAN HEMATOPOIETIC-CELL LINES - DYNAMIC ASPECTS OF THEIR CELL-TYPE-SPECIFIC EXPRESSION
SUBTYPES OF INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR IN HUMAN HEMATOPOIETIC-CELL LINES - DYNAMIC ASPECTS OF THEIR CELL-TYPE-SPECIFIC EXPRESSION
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DOI：
10.1016/0014-5793(94)00662-8
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发表时间：
1994-08-01
期刊：
影响因子：
3.5
通讯作者：
HASEGAWA, M
中科院分区：
文献类型：
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作者：
SUGIYAMA, T;YAMAMOTOHINO, M;HASEGAWA, M
三磷酸肌醇（IP3）介导的钙信号在细胞对外界刺激的反应中起重要作用。我们最近成功地克隆了来自不同基因的三种亚型的人类对应物。使用对这些亚型受体类型特异性的cDNA序列，我们在此分析了IP（3）R亚型在刺激和未刺激的人造血细胞系中的表达谱，所述造血细胞系代表T细胞、B细胞、中性粒细胞、巨噬细胞、红细胞和巨核细胞。北方和斑点印迹分析表明，每种IP（3）R亚型在这些细胞中表达不同，并且在诱导分化的刺激下，每种细胞中的表达谱动态变化。此外，发现这些细胞中的大多数同时表达至少两种不同亚型的受体。
Inositol 1,4,5-trisphosphate (IP3)-mediated Ca2+ signaling plays important roles in cellular responses to extracellular stimuli. We recently succeeded in cloning human counterparts of the three subtypes derived from separate genes. Using the cDNA sequences type-specific to these subtype receptors, we here analyzed the expression profile of IP(3)R subtypes in stimulated and unstimulated human hematopoietic cell lines representing T cells; B cells, neutrophils, macrophages, erythrocytes and megakaryocytes. Northern and dot blot analysis showed that each IP(3)R subtype is expressed differently in these cells and that the expression profile in each cell is dynamically changed upon stimuli which induce differentiation. Moreover, most of these cells were found to simultaneously express at least two different subtype receptors.